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Species: E. coli
Genetic Insert: ZapB
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_98912 Copy
Species: E. coli
Genetic Insert: ZapA
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_98911 Copy
Species: E. coli
Genetic Insert: FtsI
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28209899
Proper citation: RRID:Addgene_98995 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Comments: The His tag in N'-FtsZ was removed. 6bp were added between the RBS and start codon of FtsZ to decrease the expression level of FstZ-GFP.
Proper citation: RRID:Addgene_98915 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pTB146; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28209899
Proper citation: RRID:Addgene_98922 Copy
Species: E. coli
Genetic Insert: ChiX
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pCDF and pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32170306
Proper citation: RRID:Addgene_174662 Copy
Species: E. coli
Genetic Insert: Scrambled sgRNA targeting sequences
Vector Backbone Description: Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35636511
Proper citation: RRID:Addgene_177812 Copy
Species: E. coli
Genetic Insert: PPK(mutant)
Vector Backbone Description: Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35862758
Proper citation: RRID:Addgene_183944 Copy
Species: E. coli
Genetic Insert: PCMV-driven hph
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169493 Copy
Species: E. coli
Genetic Insert: PCMV-driven tetracycline repressor protein class B from transposon Tn10
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169566 Copy
Species: E. coli
Genetic Insert: anti mouse secondary nanobody fused to Tn5
Vector Backbone Description: Vector Backbone:pET-29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36536148
Comments: Please visit https://doi.org/10.1101/2022.03.08.483459 for bioRxiv preprint.
Proper citation: RRID:Addgene_183637 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105839 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105844 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105845 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105843 Copy
Species: E. coli
Genetic Insert: self-targeting gRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33450024
Comments: Please note this plasmid contains Illumina 2nd step primer capture sites within the insert sequence that can affect next generation sequencing results. We suggest using sanger sequencing when working with this plasmid.
Proper citation: RRID:Addgene_124221 Copy
Species: E. coli
Genetic Insert: SpCas9-HF1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33450024
Proper citation: RRID:Addgene_124226 Copy
Species: E. coli
Genetic Insert: Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
Vector Backbone Description: Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31036827
Comments: Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_124601 Copy
Species: E. coli
Genetic Insert: rpoA-rpoB-rpoC-6xHis and rpoZ
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25665556
Comments: Plasmid pVS10 encodes the E. coli rpoA-rpoB-rpoC [His6] and
rpoZ ORFs under control of T7 promoter.
Proper citation: RRID:Addgene_104398 Copy
Species: E. coli
Genetic Insert: rpoA, B, C, and Z
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression, E. coli expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25665556
Comments: pIA900 (rpoA, B, C, and Z) encodes a C-terminally His-tagged β′ which can be removed using TEV protease. Linker with TEV-His10 cloned between NheI and StuI sites of pIA787; NheI site is gone
Proper citation: RRID:Addgene_104401 Copy
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