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Species: Homo sapiens
Genetic Insert: TNGW1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCl-neo_NHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131274
Proper citation: RRID:Addgene_36928 Copy
Species: Homo sapiens
Genetic Insert: TNGW1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCl-neo_NHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131274
Comments: There is a typo in the NAR paper that incorrectly lists the N-term truncation as AA655 (instead of the correct AA566).
Proper citation: RRID:Addgene_36925 Copy
Species: Homo sapiens
Genetic Insert: WDR5
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_36900 Copy
Species: Homo sapiens
Genetic Insert: PBGD fragment
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1; Vector Types:; Bacterial Resistance:Ampicillin
Comments: PBGD fragment is located at bp# 294-366
Proper citation: RRID:Addgene_36869 Copy
Species: Homo sapiens
Genetic Insert: RFXANK
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3UXG. http://www.thesgc.org/structures/3UXG/
Proper citation: RRID:Addgene_36902 Copy
Species: Homo sapiens
Genetic Insert: ANKRA2
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3V2O. http://www.thesgc.org/structures/3V2O/
PDB: 3V2X http://www.thesgc.org/structures/3V2X/
PDB: 3V31 http://www.thesgc.org/structures/3V31/
Proper citation: RRID:Addgene_36904 Copy
Species: Homo sapiens
Genetic Insert: RFXANK
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3V3O. http://www.thesgc.org/structures/3V3O/
Proper citation: RRID:Addgene_36905 Copy
Species: Homo sapiens
Genetic Insert: CCND1-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGGGGTACGCGCGGCGG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36854 Copy
Species: Homo sapiens
Genetic Insert: SIRT6-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCCGGGAGGCCGCACTTG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36844 Copy
Species: Homo sapiens
Genetic Insert: WTX T289A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3.1 (with Venus added); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Proper citation: RRID:Addgene_36965 Copy
Species: Homo sapiens
Genetic Insert: VHL-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCTGGATCGCGGAGGGAA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36845 Copy
Species: Homo sapiens
Genetic Insert: RUNX1-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TTCAGGAGGAAGCGATGG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36841 Copy
Species: Homo sapiens
Genetic Insert: XPA-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TAAAGCCGCCGCCTCCGG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36848 Copy
Species: Homo sapiens
Genetic Insert: WTX
Vector Backbone Description: Backbone Marker:Moon Lab Plasmid available from Addgene (plasmid #15100); Backbone Size:5500; Vector Backbone:pGLUE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17510365
Comments: Learn more about the pGLUE tandem-affinity purification plasmid backbone at http://www.addgene.org/15100/ .
Proper citation: RRID:Addgene_36956 Copy
Species: Homo sapiens
Genetic Insert: EglN2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6886; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36950 Copy
Species: Homo sapiens
Genetic Insert: EglN3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36951 Copy
Species: Homo sapiens
Genetic Insert: WTX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17510365
Proper citation: RRID:Addgene_36958 Copy
Species: Homo sapiens
Genetic Insert: DNMT3B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15861382
Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI restriction site (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917.
The full-length cDNA for human DNMT3B2 was cloned into EcoRI and BamHI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen.
Proper citation: RRID:Addgene_36942 Copy
Species: Homo sapiens
Genetic Insert: Thioredoxin 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22430737
Proper citation: RRID:Addgene_36944 Copy
Species: Homo sapiens
Genetic Insert: Thioredoxin-1 C35S mutation
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22430737
Proper citation: RRID:Addgene_36947 Copy
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