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Vector Backbone Description: Backbone Size:4470; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.85, Ligation number NONE.
Proper citation: RRID:Addgene_1498 Copy
Species: Homo sapiens
Genetic Insert: LA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5933; Vector Backbone:pTracer-EF/V5-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15003 Copy
Species: Homo sapiens
Genetic Insert: TrkA
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10679771
Comments: The mammalian expression plasmid for trkA was generated by subcloning of TrkA full-length cDNA into pCMV5 vector at the EcoRI site.
Proper citation: RRID:Addgene_15002 Copy
Species: Rattus norvegicus
Genetic Insert: p130cas
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11314030
Proper citation: RRID:Addgene_15001 Copy
Species: HIV-1
Genetic Insert: Capsid
Vector Backbone Description: Backbone Marker:Bio-Rad; Backbone Size:2863; Vector Backbone:pTZ19U; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33033190
Proper citation: RRID:Addgene_149687 Copy
Species: Homo sapiens
Genetic Insert: HNF4A-FOXA2-FOXA3
Vector Backbone Description: Backbone Marker:GeneCopoeia; Backbone Size:5784; Vector Backbone:CmiR0001-MR03; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33461059
Proper citation: RRID:Addgene_149724 Copy
Species: Mus musculus
Genetic Insert: IFT88
Vector Backbone Description: Backbone Size:9800; Vector Backbone:pEF1α-IRES-EGFP (IRES-EGFP removed); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_149697 Copy
Species: Homo sapiens
Genetic Insert: PLAP
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP (A); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8455633
Comments: In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(A) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3').
Proper citation: RRID:Addgene_15160 Copy
Species: Homo sapiens
Genetic Insert: PLAP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:1731342
Comments: Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.
Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.
Proper citation: RRID:Addgene_15164 Copy
Vector Backbone Description: Backbone Size:4835; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD99.44, Ligation number L2680.
Proper citation: RRID:Addgene_1516 Copy
Species: Mus musculus
Genetic Insert: Notch1 (C-term)
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9006984
Comments: mNIC encodes the cytoplasmic domain of mouse Notch 1 with a myc tag at the N terminus.
Proper citation: RRID:Addgene_15131 Copy
Species: Homo sapiens
Genetic Insert: IRAK1 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16885212
Comments: Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of IRAK1 with 4-bp substitutions in the miR-146 target sites.
Proper citation: RRID:Addgene_15096 Copy
Vector Backbone Description: Backbone Size:4919; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD97.78, Ligation number L2603.
Proper citation: RRID:Addgene_1511 Copy
Vector Backbone Description: Backbone Size:4702; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD97.82, Ligation number L2604.
Proper citation: RRID:Addgene_1512 Copy
Vector Backbone Description: Backbone Size:0; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD54.01, Ligation number pPD54_01.
Proper citation: RRID:Addgene_1507 Copy
Species: Human coronavirus OC43
Genetic Insert: Human coronavirus OC43 nucleocapsid protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: P33469
Proper citation: RRID:Addgene_151902 Copy
Species: Homo sapiens
Genetic Insert: alpha-globin EDB fibronectin minigene
Vector Backbone Description: Backbone Size:2960; Vector Backbone:pBlueScript II KS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12719375
Comments: Hybrid minigene used for splicing assay for pre-mRNA processing. Contains a unique NdeI site for subcloning sequence of interest.
Proper citation: RRID:Addgene_15125 Copy
Species: Homo sapiens
Genetic Insert: TRAF6 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16885212
Comments: Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of TRAF6 with 4-bp substitutions in the miR-146 target sites.
Proper citation: RRID:Addgene_15094 Copy
Species: Homo sapiens
Genetic Insert: miR-146a promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16885212
Proper citation: RRID:Addgene_15091 Copy
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV Sport 6 with U6 promoter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16426460
Comments: The pCS6/U6 vector was made by ligating an M13 PCR product from the pBS/U6 vector (from the Shi lab, Harvard Medical School) to a Rsr II-Mlu I blunted pCMV-Sport6 vector (Invitrogen). This vector is compatible with Invitrogen's Gateway system.
See Author's Map for cloning information and the article for a protocol for cloning hairpins into this vector (in the article's supplementary material).
Proper citation: RRID:Addgene_15173 Copy
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