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Species: Mus musculus
Genetic Insert: TCRA
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/466243v5 for bioRxiv preprint.
Proper citation: RRID:Addgene_131033 Copy
Species: Mus musculus
Genetic Insert: -800 - 0 of mTrib2 upstream of gene transcriptional start site
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24516045
Comments: The 800 bp promoter region was cloned by digesting the T2P 2.6 plasmid with KpnI and re-ligating the plasmid with 800bp of the T2P 2.6 still attached to it.
The insert contains a A200T mismatch. This is within a putative CTTTGCAAT CEBP binding site which has not been experimentally validated, and without experimentally validated E2F binding sites.
Proper citation: RRID:Addgene_131147 Copy
Species: Mus musculus
Genetic Insert: -500 - 0 of mTrib2 upstream of gene transcriptional start site
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24516045
Proper citation: RRID:Addgene_131150 Copy
Species: Mus musculus
Genetic Insert: EED-KO-gRNA-2
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29932905
Proper citation: RRID:Addgene_131326 Copy
Species: Mus musculus
Genetic Insert: JARID2-KO-gRNA-2
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29932905
Proper citation: RRID:Addgene_131327 Copy
Species: Mus musculus
Genetic Insert: i-MT-r.Donor
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:3500; Vector Backbone:pCR™-Blunt vector; Vector Types:Mouse Targeting, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29932905
Proper citation: RRID:Addgene_131325 Copy
Species: Mus musculus
Genetic Insert: EED-KO-gRNA-2
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29932905
Proper citation: RRID:Addgene_131322 Copy
Species: Mus musculus
Genetic Insert: GATA1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15229217
Proper citation: RRID:Addgene_13626 Copy
Species: Mus musculus
Genetic Insert: PLK5
Vector Backbone Description: Vector Backbone:pcDNA3.1-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21245385
Proper citation: RRID:Addgene_136316 Copy
Species: Mus musculus
Genetic Insert: PLEKHG6
Vector Backbone Description: Vector Backbone:pENTR_D/Topo; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28692064
Proper citation: RRID:Addgene_136327 Copy
Species: Mus musculus
Genetic Insert: PLEKHG6
Vector Backbone Description: Vector Backbone:pENTR_D/Topo; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28692064
Proper citation: RRID:Addgene_136328 Copy
Species: Mus musculus
Genetic Insert: CEP55
Vector Backbone Description: Vector Backbone:pENTR_D-topo; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30069007
Proper citation: RRID:Addgene_136333 Copy
Species: Mus musculus
Genetic Insert: CCCNB1
Vector Backbone Description: Vector Backbone:pENTR_D-topo; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_136340 Copy
Species: Mus musculus
Genetic Insert: PTTG1
Vector Backbone Description: Vector Backbone:pENTR_D-topo; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_136342 Copy
Species: Mus musculus
Genetic Insert: PTTG1
Vector Backbone Description: Vector Backbone:pLenti-CMVtight-Hygro-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_136346 Copy
Species: Mus musculus
Genetic Insert: U6_sgRNA(Myb)_U6_sgRNA(Qk)
Vector Backbone Description: Backbone Marker:Addgene #50946; Vector Backbone:pKLV-U6gRNA-PGKpuro2ABFP; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29654229
Proper citation: RRID:Addgene_136415 Copy
Species: Mus musculus
Genetic Insert: PLK5
Vector Backbone Description: Vector Backbone:pcDNA3.1-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21245385
Proper citation: RRID:Addgene_136318 Copy
Species: Mus musculus
Genetic Insert: Oct4-P2A-Sox2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136576 Copy
Species: Mus musculus
Genetic Insert: Sox2-E2A-cMyc
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136578 Copy
Species: Mus musculus
Genetic Insert: Sox2-T2A-Klf4-E2A-cMyc
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136551 Copy
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