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Species: Mus musculus
Genetic Insert: beta2-YFP-M
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14684858
Comments: YFP with an upstream HA epitope tag was inserted into PpuMI restriction site of the (M3-M4) loop at residue 381 (beta2-YFP-M).
Proper citation: RRID:Addgene_15107 Copy
Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64TNE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: To make mRNA for the full length BMP receptor. The clone contains no untranslated sequences.
Proper citation: RRID:Addgene_15068 Copy
Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: To make RNA for truncated dominant negative form of RU1. The truncation is at one amino acid past the transmembrane domain.
Proper citation: RRID:Addgene_15067 Copy
Species: Xenopus laevis
Genetic Insert: BMP2-Vg1
Vector Backbone Description: Backbone Marker:Modified form of pSP64T that contains KpnI & XhoI near BglII; Backbone Size:3000; Vector Backbone:pSP64T3; Vector Types:Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8348610
Comments: PCR amplified Pro domain of Xenopus BMP2 including tetrabasic cleavage site and 4 a.a. downstream, fused to PCR fragment of Vg1 ligand domain (without first 3 a.a.).
See article for more cloning details.
The plasmid was linearized with EcoRI prior to in vitro synthesis of synthetic capped mRNA.
Proper citation: RRID:Addgene_15071 Copy
Species: Human coronavirus NL63
Genetic Insert: Human coronavirus NL63 nucleocapsid protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: Q6Q1R8
Proper citation: RRID:Addgene_151957 Copy
Species: Mus musculus
Genetic Insert: PIAS3
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pCMV-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9388184
Comments: Please note that Addgene's sequencing results identified a V580I difference when compared to GenBank reference sequence AAB88902.2. The depositing laboratory has confirmed that plasmid this is exact construct used in the associated publication.
Proper citation: RRID:Addgene_15207 Copy
Species: Mus musculus
Genetic Insert: Chx10 3kb AP reporter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15893976
Comments: The 3Kb-AP reporter was constructed using a
genomic BamHI–SacII fragment isolated from a Chx10
BAC (Rowan and Cepko, 2004) and subcloned into pBluescript
(Stratagene). An SV40 intervening sequence (IVS)
was isolated from pNASSbeta (Clontech) digested with EcoRI
and blunt cloned into the 3' SacII restriction site. The full length alkaline phosphatase (AP) reporter construct was
isolated from the SV40all plasmid (gift of S. Dymecki).
Proper citation: RRID:Addgene_15205 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 N (Nucleocapsid)
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: QHN73817.1
Proper citation: RRID:Addgene_151966 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp2
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725298.1
Proper citation: RRID:Addgene_152022 Copy
Species: Human coronavirus HKU1
Genetic Insert: Human coronavirus HKU1 nucleocapsid phosphoprotein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: Q5MQC6
Proper citation: RRID:Addgene_151948 Copy
Species: Homo sapiens
Genetic Insert: Human neuronal kinesin heavy chain
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9244295
Proper citation: RRID:Addgene_15219 Copy
Species: Gallus gallus
Genetic Insert: Paxillin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16254239
Proper citation: RRID:Addgene_15212 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724390.1
Proper citation: RRID:Addgene_152113 Copy
Vector Backbone Description: Backbone Size:9726; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD103.05, Ligation number L2865.
Proper citation: RRID:Addgene_1522 Copy
Species: Mus musculus
Genetic Insert: Neuroligin 1 with insert A
Vector Backbone Description: Backbone Size:4842; Vector Backbone:pCAAGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16846852
Proper citation: RRID:Addgene_15227 Copy
Species: Homo sapiens
Genetic Insert: G protein coupled receptor kinase-interacting protein 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:PEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11896197
Proper citation: RRID:Addgene_15226 Copy
Species: Homo sapiens
Genetic Insert: G protein coupled receptor kinase-interacting protein 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16825424
Proper citation: RRID:Addgene_15223 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725305.1
Proper citation: RRID:Addgene_152401 Copy
Species: Homo sapiens
Genetic Insert: Human neuronal kinesin heavy chain
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7514426
Proper citation: RRID:Addgene_15239 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724391.1
Proper citation: RRID:Addgene_152341 Copy
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