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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
PB-EEA-5g-OSK2M2L1-PGK-Puro
 
Resource Report
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1+ mentions
RRID:Addgene_102909 EEA-5g-OSK2M2L1-gRNAs-PGK-Puro Homo sapiens Ampicillin PMID:29980666 Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:00:27 1
GG-EBNA-OSK2M2L1-PP
 
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1+ mentions
RRID:Addgene_102902 OSK2M2L1-gRNAs-PGK-puro Other Ampicillin PMID:29980666 Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:00:27 1
pLEX307-mFzd5
 
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1+ mentions
RRID:Addgene_102867 Frizzled 5 Mus musculus Ampicillin PMID:28885975 Backbone Marker:Addgene #41392 (provided by David Root); Backbone Size:10065; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin A to G substitution at nucleotide 699 of Fzd5 ORF; the substitution is silent and does not alter the amino acid sequence 2026-08-15 01:00:27 1
pLEX307-mFzd1
 
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1+ mentions
RRID:Addgene_102864 Frizzled 1 Mus musculus Ampicillin PMID:28885975 Backbone Marker:Addgene #41392 (provided by David Root); Backbone Size:10065; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:00:27 1
pCMV-ABE7.9
 
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1+ mentions
RRID:Addgene_102918 ABE7.9 Synthetic Ampicillin PMID:29160308 Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin see manuscript 2026-08-15 01:00:27 3
pCMV-ABE7.10
 
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10+ mentions
RRID:Addgene_102919 ABE7.10 Synthetic Ampicillin PMID:29160308 Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin see manuscript 2026-08-15 01:00:27 40
STAGR_SAMScaffold_hU6
 
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1+ mentions
RRID:Addgene_102843 gRNAScaffold_hU6promoter Kanamycin PMID:29702666 Backbone Size:3250; Vector Backbone:Human gRNA Expression Vector / PCR Template for STAgR; Vector Types:as PCR template; Bacterial Resistance:Kanamycin 2026-08-15 01:00:27 1
STAGR_gRNAScaffold_mU6
 
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1+ mentions
RRID:Addgene_102844 STAgR Insert gRNAScaffold_mU6 Kanamycin PMID:29702666 Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin 2026-08-15 01:00:26 2
hisSUMO-CTCF(F1-F9)
 
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1+ mentions
RRID:Addgene_102859 CTCF Mus musculus Ampicillin PMID:29159284 This plasmid encodes the DNA binding domain of mouse CTCF protein (F1 to F9) for E. coli expression. Vector Backbone:NEB DHFR control plasmid; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:26 1
pCMV-5U-Venus-PSD-95-3U
 
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1+ mentions
RRID:Addgene_102949 PSD-95 Mus musculus Kanamycin PMID:25948262 Backbone Size:4141; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:00:27 3
PresentER-SIINFEKL (mCherry)
 
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1+ mentions
RRID:Addgene_102945 SIINFEKL Gallus gallus Ampicillin PMID:30499773 Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint. Backbone Size:8200; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin OVA257-264 2026-08-15 01:00:27 4
pET22b_PfSSB
 
Resource Report
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1+ mentions
RRID:Addgene_103000 PfSSB Plasmodium falciparum Ampicillin PMID:29466468 The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, for subsequent labeling at the single, native cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~20-fold fluorescence increase on binding ssDNA with very high affinity. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77. Protocol See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 1
pUCmini-iCAP-PHP.B2
 
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1+ mentions
RRID:Addgene_103003 Synthetic construct isolate AAV-PHP.B2 VP1 gene Synthetic Ampicillin PMID:26829320 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 1
pUCmini-iCAP-PHP.B
 
Resource Report
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10+ mentions
RRID:Addgene_103002 Synthetic construct isolate AAV-PHP.B VP1 gene Synthetic Ampicillin PMID:26829320 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 14
pUCmini-iCAP-PHP.eB
 
Resource Report
Resource Website
100+ mentions
RRID:Addgene_103005 Synthetic construct isolate AAV-PHP.eB VP1 gene Synthetic Ampicillin PMID:28671695 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 135
pUCmini-iCAP-PHP.B3
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_103004 Synthetic construct isolate AAV-PHP.B3 VP1 gene Synthetic Ampicillin PMID:26829320 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 1
pCAGGS_3E5E_luciferase
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_103055 Ebolavirus minigenome Zaire ebolavirus, Mayinga variant Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 2
pSC101_TIMER
 
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1+ mentions
RRID:Addgene_103057 TIMER-bac Synthetic Kanamycin PMID:25126781 TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance. Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin mutations: S4T and S179T 2026-08-15 01:00:29 4
pCAGGS_L_EBOV
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_103052 L gene of Ebola virus Zaire ebolavirus, Mayinga Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 4
pCAGGS_VP30_EBOV
 
Resource Report
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1+ mentions
RRID:Addgene_103051 VP30 gene of Ebola virus Zaire ebolavirus, Mayinga Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 3

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