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Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFC2
Vector Backbone Description: Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258208 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABEmax
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The depositor confirms that G5A in EGFP in the Addgene sequence is expected and does not alter the plasmid's function. Please refer to the the Addgene sequence for the most accurate result.
Proper citation: RRID:Addgene_251965 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Myc-tagged Sed1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO^NIT
Vector Backbone Description: Backbone Size:4665; Vector Backbone:pLANT-2b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_231487 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 ILVtoED
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231857 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231855 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 ILVtoAS
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231859 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 R2
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231870 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 R3 W
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231876 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 L123A+
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231869 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Swap3
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231860 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Swap2
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231865 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 WTSLFD
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231881 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 ILVtoED solvvol
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231858 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE13 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232887 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VHT1 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232898 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2 gRNA and repair template
Vector Backbone Description: Vector Backbone:pCRISPEY-GAL-Nat; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232103 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AVO1 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232889 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rat1
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683646
Proper citation: RRID:Addgene_231650 Copy
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