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Species: Synthetic
Genetic Insert: (10 X tetO)-t
Vector Backbone Description: Backbone Size:2672; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33235249
Comments: This plasmid may be prone to recombination. It is recommended to screen multiple DNA preparations from cultures started from single colonies.
Proper citation: RRID:Addgene_160825 Copy
Species: Synthetic
Genetic Insert: (40 X tetO)-t
Vector Backbone Description: Backbone Size:2672; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33235249
Comments: This plasmid may be prone to recombination. It is recommended to screen multiple DNA preparations from cultures started from single colonies.
Proper citation: RRID:Addgene_160827 Copy
Species: Synthetic
Genetic Insert: (80 X tetO)-t
Vector Backbone Description: Backbone Size:2672; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33235249
Comments: This plasmid may be prone to recombination. It is recommended to screen multiple DNA preparations from cultures started from single colonies.
Proper citation: RRID:Addgene_160828 Copy
Species: Synthetic
Genetic Insert: (160 X tetO)-t
Vector Backbone Description: Backbone Size:2672; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33235249
Comments: This plasmid may be prone to recombination. It is recommended to screen multiple DNA preparations from cultures started from single colonies.
Proper citation: RRID:Addgene_160829 Copy
Species: E. coli
Genetic Insert: HSP33 cys
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5190; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16054089
Comments: The HSP-FRET probe was generated by inserting YFP into the pECFP-N1 plasmid between the NheI and BglII sites and then ligating the redox-sensitive regulatory domain from the Escherichia coli HSP-33 between YFP and CFP via the EcoRI and BamHI sites. HSP-33: 210 amino acids; BA000007 region 4241962-4242826. Made in Hammerling lab; used in Waypa et al., Increases in mitochondrial reactive oxygen species trigger hypoxia-induced calcium responses in pulmonary artery smooth muscle cells, Circ Res. 2006 Oct 27; 99(9):970-8 PMID: 17008601.
Proper citation: RRID:Addgene_16076 Copy
Genetic Insert: VioA
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158598 Copy
Genetic Insert: tNCS-ePTS1
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158595 Copy
Genetic Insert: tNCS-VioE-dead_ePTS1
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158592 Copy
Genetic Insert: tNCS-Venus-ePTS1
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158590 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADR1c
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158600 Copy
Species: Synthetic
Genetic Insert: phoAp Griffithsin
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1788; Vector Backbone:pSMART HCKan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32974328
Proper citation: RRID:Addgene_158747 Copy
Species: Synthetic
Genetic Insert: yibDp Griffithsin
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1788; Vector Backbone:pSMART HCKan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32974328
Proper citation: RRID:Addgene_158745 Copy
Species: M. marinum
Genetic Insert: recX
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31992616
Comments: 5' Cloning Site: KpnI (destroyed), 3' Cloning Site: KpnI (destroyed)
Proper citation: RRID:Addgene_158718 Copy
Species: M. marinum
Genetic Insert: recA
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31992616
Comments: 5' Cloning Site: KpnI (destroyed), 3' Cloning Site: KpnI (destroyed)
Proper citation: RRID:Addgene_158717 Copy
Species: Streptococcus thermophilus
Genetic Insert: cas9
Vector Backbone Description: Vector Backbone:pNHEJ-Cas12a-recX; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31992616
Proper citation: RRID:Addgene_158715 Copy
Species: Francisella tularensis subsp. Novicida
Genetic Insert: AcGFP (for crRNA cloning)
Vector Backbone Description: Vector Backbone:Pacyc184; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28646112
Proper citation: RRID:Addgene_158712 Copy
Species: M. marinum
Genetic Insert: the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
Vector Backbone Description: Vector Backbone:pJV53-Cas12a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31992616
Comments: 5' Cloning Site: KpnI (not destroyed), 3' Cloning Site: NheI (destroyed)
Proper citation: RRID:Addgene_158713 Copy
Species: Other
Genetic Insert: dCasPhi-3 (D413A)
Vector Backbone Description: Vector Backbone:pRSF-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32675376
Comments: Please note there is a silent mutation in the dCasPhi-3 coding sequence that does not alter plasmid function.
Proper citation: RRID:Addgene_158799 Copy
Species: Other
Genetic Insert: CasPhi-3
Vector Backbone Description: Vector Backbone:pRSF-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32675376
Proper citation: RRID:Addgene_158796 Copy
Species: Other
Genetic Insert: CasPhi-1
Vector Backbone Description: Vector Backbone:pRSF-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32675376
Proper citation: RRID:Addgene_158794 Copy
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