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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCDNA3-Flag-SPRTN-E112A Resource Report Resource Website 1+ mentions |
RRID:Addgene_110215 | SPRTN | Homo sapiens | Ampicillin | PMID:25261934 | According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant. | Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | E112A, P296L (see depositor comments below) | 2026-08-15 01:01:38 | 1 |
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pCDNA3-Flag-SPRTN-Y117C Resource Report Resource Website |
RRID:Addgene_110216 | SPRTN | Homo sapiens | Ampicillin | PMID:27871366 | According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant. | Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Y117C, P296L (see depositor comments below) | 2026-08-15 01:01:38 | 0 |
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pCDNA3-Flag-SPRTN-wt Resource Report Resource Website 1+ mentions |
RRID:Addgene_110214 | SPRTN | Homo sapiens | Ampicillin | PMID:25261934 | According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant. | Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | P296L (see depositor comments below) | 2026-08-15 01:01:38 | 1 |
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pNIC-ZB-SPRTN-Y117C-FL Resource Report Resource Website |
RRID:Addgene_110219 | SPRTN | Homo sapiens | Kanamycin | PMID:27871366 | According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant. | Backbone Marker:Opher Gileadi, Addgene plasmid #26107; Vector Backbone:pNIC-ZB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Y117C, P296L (see depositor comments below) | 2026-08-15 01:01:38 | 0 |
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DENND1A (DENND1AA-c032) Resource Report Resource Website |
RRID:Addgene_110270 | DENND1A | Homo sapiens | Ampicillin | Use SF9 cells for expression. 6EKK: https://www.thesgc.org/structures/6EKK. | Backbone Marker:Nicola Burgess-Brown (Addgene plasmid # 39191); Vector Backbone:pFB-CT10HF-LIC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:38 | 0 | ||
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ELONGIN BC (XX01TCEB1A-c001) Resource Report Resource Website 1+ mentions |
RRID:Addgene_110274 | Elongin BC | Homo sapiens | Chloramphenicol | Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1. Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins. Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK... 2C9W: https://www.thesgc.org/structures/2C9W. | Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Elongin B is full length. Elongin C contains a.a. 17-112. | 2026-08-15 01:01:39 | 4 | |
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MTHFR (MTHFRA-c042) Resource Report Resource Website |
RRID:Addgene_110271 | MTHFR | Homo sapiens | Ampicillin | Use SF9 cells for expression. PDB: 6FCX | Backbone Marker:Nicola Burgess-Brown (Addgene plasmid # 39191); Vector Backbone:pFB-CT10HF-LIC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:38 | 0 | ||
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ULK3 (ULK3A-c002) Resource Report Resource Website |
RRID:Addgene_110272 | ULK3 | Homo sapiens | Ampicillin | Use SF9 cells for expression. PDB: 6FDY | Backbone Marker:SGC Oxford; Vector Backbone:pFB-6HZB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:39 | 0 | ||
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pLKO.1-TRC.mKO2_shGLS Resource Report Resource Website |
RRID:Addgene_110319 | GLS Glutaminase | Homo sapiens | Ampicillin | PMID:29115931 | See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors. Plasmid grows more slowly than standard plasmids. | Backbone Marker:#85208; Backbone Size:7032; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:39 | 0 | |
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DPYSL4 (DPYSL4A-c003) Resource Report Resource Website |
RRID:Addgene_110263 | DPYSL4 | Homo sapiens | Ampicillin | Use SF9 cells for expression. 5NKS: https://www.thesgc.org/structures/5NKS. | Backbone Marker:SGC Oxford; Vector Backbone:pFB-6HZB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:38 | 0 | ||
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WNK3 (WNK3B-c001) Resource Report Resource Website |
RRID:Addgene_110261 | WNK3 | Homo sapiens | Ampicillin | Use SF9 cells for expression. 5O2C: https://www.thesgc.org/structures/5O2C. | Backbone Marker:Opher Gileadi (Addgene plasmid # 26108); Vector Backbone:pFB-LIC-Bse; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:38 | 0 | ||
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pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D Resource Report Resource Website |
RRID:Addgene_110300 | p53-binding protein 1 | Homo sapiens | Ampicillin | PMID:31253795 | Backbone Marker:Feng Zhang, Addgene plasmid #48139; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:39 | 0 | ||
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LIMK2 (LIMK2A-c052) Resource Report Resource Website |
RRID:Addgene_110264 | LIMK2 | Homo sapiens | Ampicillin | Use SF9 cells for expression. 5NXD: https://www.thesgc.org/structures/5NXD. | Backbone Marker:Opher Gileadi (Addgene plasmid # 26108); Vector Backbone:pFB-LIC-Bse; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:38 | 0 | ||
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ACVR1 (ACVR1Z-c041) Resource Report Resource Website |
RRID:Addgene_110269 | ACVR1 | Homo sapiens | Ampicillin | Use SF9 cells for expression. 6EIX: https://www.thesgc.org/structures/6EIX. | Backbone Marker:Opher Gileadi (Addgene plasmid # 26108); Vector Backbone:pFB-LIC-Bse; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:01:38 | 0 | ||
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WNK2 (WNK2A-c003) Resource Report Resource Website |
RRID:Addgene_110250 | WNK2 | Homo sapiens | Kanamycin | Use BL21(DE3)-R3-pRARE2 for expression. 6ELM: https://www.thesgc.org/structures/6ELM. | Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:01:38 | 0 | ||
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KALRN (KALRNA-c001) Resource Report Resource Website |
RRID:Addgene_110256 | KALRN | Homo sapiens | Kanamycin | Use BL21 (DE3) Star for expression. PDB structure is a complex of (KALRNA-c001 & RAC1A-c007). 5o33: https://www.thesgc.org/structures/5o33. | Vector Backbone:pNIC28-10His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:01:38 | 0 | ||
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PARP14 (PARP14A-c013) Resource Report Resource Website |
RRID:Addgene_110254 | PARP14 | Homo sapiens | Kanamycin | Use BL21(DE3)-R3-pRARE2 for expression. 5O2D: https://www.thesgc.org/structures/5O2D. | Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:01:38 | 0 | ||
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IQSEC2 (IQSEC2A-c001) Resource Report Resource Website |
RRID:Addgene_110259 | IQSEC2 | Homo sapiens | Kanamycin | Use BL21 (DE3) Star for expression. PDB structure, 6FAE, is a complex of (ARF1A-c001 & IQSEC2A-c003). | Vector Backbone:pNIC28-10His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:01:38 | 0 | ||
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TRMT10C (RG9MTD1A-c113) Resource Report Resource Website |
RRID:Addgene_110248 | RG9MTD1 | Homo sapiens | Kanamycin | Use BL21(DE3)-R3-pRARE2 for expression. 5NFJ: https://www.thesgc.org/structures/5NFJ. | Backbone Marker:Opher Gileadi (Addgene plasmid # 26105); Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:01:38 | 0 | ||
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FOXN1 (FOXN1A-c017) Resource Report Resource Website |
RRID:Addgene_110249 | FOXN1 | Homo sapiens | Kanamycin | Use BL21(DE3)-R3-pRARE2 for expression. 5OCN: https://www.thesgc.org/structures/5OCN. | Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:01:38 | 0 |
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