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Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Marker:available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9032279
Proper citation: RRID:Addgene_11025 Copy
Species: Homo sapiens
Genetic Insert: KGA glutaminase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3.1D/V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22228304
Proper citation: RRID:Addgene_110332 Copy
Species: Homo sapiens
Genetic Insert: B3GNT5
Vector Backbone Description: Backbone Marker:#85208; Backbone Size:7032; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29115931
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110325 Copy
Species: Homo sapiens
Genetic Insert: HRASLS
Vector Backbone Description: Backbone Marker:#85208; Backbone Size:7032; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29115931
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110324 Copy
Species: Homo sapiens
Genetic Insert: KGA glutaminase
Vector Backbone Description: Backbone Marker:#110344; Backbone Size:7423; Vector Backbone:pQC MCS IRES G418; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: KGA.wt obtained with V5-6xHis from pcDNA3.1D V5-His-TOPO-KGA.wt (Addgene #110332)
pQC-based γ-Retroviral transfer vector for KGA.wt-V5 expression. IRES-driven Puromycin selection.
Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110390 Copy
Species: Homo sapiens
Genetic Insert: ELAV like RNA binding protein 1
Vector Backbone Description: Backbone Marker:#110341; Backbone Size:7757; Vector Backbone:pQC V5 mKO2 MCS IRES Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: pQC-based γ-Retroviral transfer vector for V5-mKO2-HuR.wt expression. IRES-driven Puromycin selection.
Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110387 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:#26655; Backbone Size:6783; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110420 Copy
Species: Homo sapiens
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110421 Copy
Species: Homo sapiens
Genetic Insert: ELAV like RNA binding protein 1
Vector Backbone Description: Backbone Marker:#110342; Backbone Size:7064; Vector Backbone:pQC V5 MCS IRES Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: pQC-based γ-Retroviral transfer vector for V5-HuR.wt expression. IRES-driven Puromycin selection.
Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110386 Copy
Species: Homo sapiens
Genetic Insert: ELAVL1 HuR
Vector Backbone Description: Backbone Marker:#85208; Backbone Size:7032; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110426 Copy
Species: Homo sapiens
Genetic Insert: ELAV like RNA binding protein 1
Vector Backbone Description: Backbone Marker:#110371; Backbone Size:5834; Vector Backbone:pcDNA5/FRT/TO/FLAG-mKO2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Insert created by joining regions before (Addgene #110395) and after (Addgene #110395) HuR HNS by HindIII restriction site
Proper citation: RRID:Addgene_110373 Copy
Species: Homo sapiens
Genetic Insert: Neurogenin 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:Mammalian Expression, Cre/Lox, CRISPR, TALEN; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110492 Copy
Species: Homo sapiens
Genetic Insert: Yes
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110497 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:#26655; Backbone Size:6783; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110410 Copy
Species: Homo sapiens
Genetic Insert: ELAVL1 (HuR)
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110411 Copy
Species: Homo sapiens
Genetic Insert: ELAVL1 (HuR)
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110412 Copy
Species: Homo sapiens
Genetic Insert: HuR (ELAVL1)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3.1D/V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110417 Copy
Species: Homo sapiens
Genetic Insert: HRPT2 136X
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15632063
Comments: Author has deposited insert sequence. Click on "sequence" to view. Cloned using the following primers 5' CGGGATCCATGGCGGACGTGCTTAGCGT 3' and 5' CCGCTCGAGCTATGCTTCTGCTAAAACTTC 3' and ligated into pcDNA3 HRPT2 that had been digested with BamHI & XhoI (which removes full-length HRPT2, but leaves the flag tag at the N-terminus. See plasmid 11048 to learn how pcDNA3 HRPT2 was constructed).
Proper citation: RRID:Addgene_11049 Copy
Species: Homo sapiens
Genetic Insert: HRPT2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15632063
Comments: Author has deposited insert sequence. Click on "sequence" to view. Cloned using the following primers 5' CGGAATTCGGATCCACCATGGATTACAAGGATGACGACGATAAGGTCGACATGGCGGACGTGCTTAGCGT 3' and 5' CCGCTCGAGTCAGAATCTCAAGTGCGATTTATGCTT 3'
Proper citation: RRID:Addgene_11048 Copy
Species: Homo sapiens
Genetic Insert: SOX4
Vector Backbone Description: Backbone Marker:William Sellers; Vector Backbone:pcDNA3-Flag-FKHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16618720
Proper citation: RRID:Addgene_110360 Copy
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