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Species: Saccharomyces cerevisiae
Genetic Insert: nArg8-EGFP
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Proper citation: RRID:Addgene_251977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41794024
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227762 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tMsk1
Vector Backbone Description: Vector Backbone:pB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Proper citation: RRID:Addgene_251971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS38
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42207670
Proper citation: RRID:Addgene_235768 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Backbone plasmid for subcloning
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41841725
Proper citation: RRID:Addgene_242948 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS15
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42207670
Proper citation: RRID:Addgene_235723 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS15
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42207670
Proper citation: RRID:Addgene_235722 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABE8e
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Proper citation: RRID:Addgene_251975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABE8e
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Proper citation: RRID:Addgene_251974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Leaf compost cutinase
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253049 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NcoI_SMT3-SUMO(21-98)_C_sTEV_His6
Vector Backbone Description: Backbone Size:5237; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41927759
Comments: This vector is a modified pET29b(+) vector that includes one added cloning site (NcoI) followed by the encoding region for a fragment of the SMT3_yeast gene (21-98) followed by a TEV site (ENLYFQS) and a hexa-histidine site (HHHHHH*). Any peptide of interest can be cloned in the vector using the two cloning sites (NdeI and NcoI) via HiFi assembly. When adding a peptide of interest this way, the remaining N-terminal portion of the SMT3 gene should be added as well, separated by the peptide of interest with a GSGS encoding region (GSGSMSDSEVNQEAKPEVKPEVKP). This can result in a vector which encodes for the expression of a recombinant gene of the following type : PEPTIDE-GSGS-SUMO(1-98)-C-sTEV_His6*.
Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint.
Proper citation: RRID:Addgene_254142 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLAG-tagged Sed1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252976 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253027 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABEm-Arg8m*
Vector Backbone Description: Vector Backbone:pPT24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Insert is flanked by the 5' and 3'UTR of COX2 and the complete COX2 gene further downstream. Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory by 70 bp inserted between the ‘F0’ and ‘F1’ features in the annotated sequence map. The depositor confirms that this insertion is expected and corresponds to the 5’ UTR of COX2.
Proper citation: RRID:Addgene_251983 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mtArg8m*
Vector Backbone Description: Vector Backbone:pPT24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Insert is flanked by the 5' and 3'UTR of COX2 and the complete COX2 gene further downstream. Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory that changes amino acids #3002-3005 from SKKV to IEEG in the insert ORF. The depositor confirms that the Addgene result is correct.
Proper citation: RRID:Addgene_251980 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2 Protein A
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253044 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERO1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HAC1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252991 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MSN4
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252992 Copy
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