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Species: Saccharomyces cerevisiae
Genetic Insert: ADE2 gRNA and repair template
Vector Backbone Description: Vector Backbone:pCRISPEY-Z3-Nat; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: Gibson cloning at XhoI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232106 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2 gRNA and repair template
Vector Backbone Description: Vector Backbone:pCRISPEY-GAL-Kan; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE2 gRNA and repair template
Vector Backbone Description: Vector Backbone:pCRISPEY-GAL-Hyg; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40758833
Comments: Gibson cloning at NotI sites . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.
Proper citation: RRID:Addgene_232104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: spt5
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683646
Proper citation: RRID:Addgene_231648 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3 gRNA
Vector Backbone Description: Backbone Marker:Patricia Wittkopp; Backbone Size:11961; Vector Backbone:pML104-HygMX4; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232885 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RER2 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12368; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232884 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:11240; Vector Backbone:pML104; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232894 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5H28
Vector Backbone Description: Backbone Size:5679; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42555168
Comments: 5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)
Proper citation: RRID:Addgene_234184 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MFA-IgKappa-FAPoptim-STE3-deltaPEST
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: Amino acid 413 was mutated to a stop codon using site-directed mutagenesis, creating a truncation mutant of Ste3s C-tail at this residue in the pRS415-STE3pr-MFA-Igkappa-FAP-STE3.
Proper citation: RRID:Addgene_221118 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ANP1-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of ANP1 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221120 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ARB1 gRNA
Vector Backbone Description: Backbone Marker:Laughery et al, 2015, Yeast; Backbone Size:12367; Vector Backbone:pML107; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41775267
Proper citation: RRID:Addgene_232888 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Prp8
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234120 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERO1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HAC1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252991 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MSN4
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252992 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PDI1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252993 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSO1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252996 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERJ5
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252989 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MYC-tagged S. cerevisiae Aga1 del N181aa display anchor
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252983 Copy
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