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nArg8-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_251977 nArg8-EGFP Saccharomyces cerevisiae Ampicillin PMID:41543903 Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint. Vector Backbone:pAG415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:11 0
pVG298
 
Resource Report
Resource Website 		RRID:Addgene_227762 EL222 codon-optimized for budding yeast Saccharomyces cerevisiae Ampicillin PMID:41794024 For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint. Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Thr83Ala mutant 2026-08-15 01:35:11 0
pBC-tMsk1
 
Resource Report
Resource Website 		RRID:Addgene_251971 tMsk1 Saccharomyces cerevisiae Ampicillin PMID:41543903 Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint. Vector Backbone:pB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin deleted aa 1-26 2026-08-15 01:35:13 0
pYO1922
 
Resource Report
Resource Website 		RRID:Addgene_235768 VPS38 Saccharomyces cerevisiae Ampicillin PMID:42207670 Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:17 0
pRS313 Gal1p2
 
Resource Report
Resource Website 		RRID:Addgene_242948 Backbone plasmid for subcloning Saccharomyces cerevisiae Ampicillin PMID:41841725 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:17 0
pYO1877
 
Resource Report
Resource Website 		RRID:Addgene_235723 VPS15 Saccharomyces cerevisiae Ampicillin PMID:42207670 Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin S622D, H623D, T626E 2026-08-15 01:35:17 0
pYO950
 
Resource Report
Resource Website 		RRID:Addgene_235722 VPS15 Saccharomyces cerevisiae Ampicillin PMID:42207670 Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:17 0
MTS-ABE8e(∆REC2)-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_251975 ABE8e Saccharomyces cerevisiae Ampicillin PMID:41543903 Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint. Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin REC2 deleted 2026-08-15 01:35:45 0
MTS-ABE8e(∆HNH)-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_251974 ABE8e Saccharomyces cerevisiae Ampicillin PMID:41543903 Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint. Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin HNH deleted 2026-08-15 01:35:45 0
pYSD726
 
Resource Report
Resource Website 		RRID:Addgene_253049 Leaf compost cutinase Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Y127G, D238C, F243I, S283C 2026-08-15 01:35:45 0
pET29b(+)_NdeI-NcoI_SUMO(21-98)_CV
 
Resource Report
Resource Website 		RRID:Addgene_254142 NcoI_SMT3-SUMO(21-98)_C_sTEV_His6 Saccharomyces cerevisiae Kanamycin PMID:41927759 This vector is a modified pET29b(+) vector that includes one added cloning site (NcoI) followed by the encoding region for a fragment of the SMT3_yeast gene (21-98) followed by a TEV site (ENLYFQS) and a hexa-histidine site (HHHHHH*). Any peptide of interest can be cloned in the vector using the two cloning sites (NdeI and NcoI) via HiFi assembly. When adding a peptide of interest this way, the remaining N-terminal portion of the SMT3 gene should be added as well, separated by the peptide of interest with a GSGS encoding region (GSGSMSDSEVNQEAKPEVKPEVKP). This can result in a vector which encodes for the expression of a recombinant gene of the following type : PEPTIDE-GSGS-SUMO(1-98)-C-sTEV_His6*. Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint. Backbone Size:5237; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Contains encoding region for enzyme site NdeI, then followed by the 21-98 AA of SMT3_yeast, followed by encoding region for a Cystein, a TEV site and a hexa-histidine encoding region. 2026-08-15 01:35:46 0
pYSD086
 
Resource Report
Resource Website 		RRID:Addgene_252976 FLAG-tagged Sed1 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:45 0
pYSD294
 
Resource Report
Resource Website 		RRID:Addgene_253027 AGA1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:45 0
pPT24-ABEm-mtArg8m*-V5
 
Resource Report
Resource Website 		RRID:Addgene_251983 ABEm-Arg8m* Saccharomyces cerevisiae Ampicillin PMID:41543903 Insert is flanked by the 5' and 3'UTR of COX2 and the complete COX2 gene further downstream. Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint. Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory by 70 bp inserted between the ‘F0’ and ‘F1’ features in the annotated sequence map. The depositor confirms that this insertion is expected and corresponds to the 5’ UTR of COX2. Vector Backbone:pPT24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin premature stop CAA->TAA, Q79* in Arg8 2026-08-15 01:35:45 0
pPT24-mtArg8m*-V5-UTR-HH-sgRNA-HDV
 
Resource Report
Resource Website 		RRID:Addgene_251980 mtArg8m* Saccharomyces cerevisiae Ampicillin PMID:41543903 Insert is flanked by the 5' and 3'UTR of COX2 and the complete COX2 gene further downstream. Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint. Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory that changes amino acids #3002-3005 from SKKV to IEEG in the insert ORF. The depositor confirms that the Addgene result is correct. Vector Backbone:pPT24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin premature stop CAA->TAA, Q79* 2026-08-15 01:35:45 0
pYSD518
 
Resource Report
Resource Website 		RRID:Addgene_253003 SSA1 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:45 0
pYSD344
 
Resource Report
Resource Website 		RRID:Addgene_253044 Aga2 Protein A Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:48 0
pYSD505
 
Resource Report
Resource Website 		RRID:Addgene_252990 ERO1 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD506
 
Resource Report
Resource Website 		RRID:Addgene_252991 HAC1 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD507
 
Resource Report
Resource Website 		RRID:Addgene_252992 MSN4 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0

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