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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp12
Vector Backbone Description: Backbone Size:6610; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32438371
Proper citation: RRID:Addgene_154759 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (HexaPro variant)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32703906
Proper citation: RRID:Addgene_154754 Copy
Species: Methanosarcina mazei
Genetic Insert: Mma PylRS
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30260624
Comments: 5' Cloning Site: NheI; 3' Cloning Site: SalI.
Proper citation: RRID:Addgene_154762 Copy
Species: Homo sapiens
Genetic Insert: coEPO
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:2703; Vector Backbone:pJ204; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32835482
Comments: This is a donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE) using poly(A)-trap.
Promoter 100RPU.2 was initially described in:
Brown et al. 2017 "In silico design of context-responsive mammalian promoters with user-defined functionality." DOI: 10.1093/nar/gkx768
A patent application has been filed by third parties on the promoter 100RPU.2 used in this plasmid, permission for deposition of plasmids containing the promoter sequence has been provided by AstraZeneca and The University of Sheffield.
The plasmid was assembled by USER cloning.
Proper citation: RRID:Addgene_154829 Copy
Species: Synthetic
Genetic Insert: Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
Vector Backbone Description: Vector Backbone:pSEVA291; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32669542
Proper citation: RRID:Addgene_154844 Copy
Species: Homo sapiens
Genetic Insert: PRAS40
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17277771
Proper citation: RRID:Addgene_15479 Copy
Species: Homo sapiens
Genetic Insert: TSC2 shRNA
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17277771
Comments: Target sequence for human TSC2 is 5'-CACTGGCCTTGGACGGTATTG-3'.
Proper citation: RRID:Addgene_15478 Copy
Genetic Insert: hM4D(Gi)-mCherry
Vector Backbone Description: Backbone Marker:Gether lab - modified from Addgene plasmid #55641; Backbone Size:4824; Vector Backbone:pAAV-hSyn-Flp-WPRE; Vector Types:Mammalian Expression, AAV, FLP-FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29938215
Proper citation: RRID:Addgene_154867 Copy
Species: Mus musculus
Genetic Insert: PRAS40 shRNA
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17277771
Comments: Target sequence for mouse PRAS40 is 5'-GAGCCCACTGAAACAGAGACA-3'.
Proper citation: RRID:Addgene_15480 Copy
Species: Methanosarcina mazei
Genetic Insert: Mma PylRS
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32579937
Comments: M15 PylT described by Serfling et al., 2018 (PMID: 29177436 )
Proper citation: RRID:Addgene_154774 Copy
Species: Synthetic
Genetic Insert: somBiPOLES
Vector Backbone Description: Backbone Size:3779; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34312384
Comments: The insert contains the fluorescence tag mCerulean3 in between the 2 channelrhodopsins (C-terminal of GtACR2) . Please visit https://www.biorxiv.org/content/10.1101/2020.07.15.204347v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_154948 Copy
Species: SARS-CoV-2
Genetic Insert: 6His-TEV-Nsp14
Vector Backbone Description: Backbone Size:5233; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_154954 Copy
Species: Synthetic
Genetic Insert: somBiPOLES
Vector Backbone Description: Backbone Size:4822; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34312384
Comments: The insert contains the fluorescence tag mCerulean3 in between the 2 channelrhodopsins (C-terminal of GtACR2) . Please visit https://www.biorxiv.org/content/10.1101/2020.07.15.204347v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_154951 Copy
Species: Xenopus laevis
Genetic Insert: Dishevelled
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939601
Comments: Xdd1 is a mutant Xdsh with a 200 bp internal deletion of the conserved PDZ/DHR domain. See author's map for details.
Proper citation: RRID:Addgene_15491 Copy
Species: Homo sapiens
Genetic Insert: Phosphoglycerate dehydrogenase
Vector Backbone Description: Backbone Size:7943; Vector Backbone:pCW57-MCS1-P2A-MCS2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32571778
Proper citation: RRID:Addgene_154903 Copy
Species: Homo sapiens
Genetic Insert: KLF2-2A-NANOG
Vector Backbone Description: Vector Backbone:pCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33798453
Proper citation: RRID:Addgene_154879 Copy
Genetic Insert: SP1-Sleeping Beauty
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32710817
Comments: Mitra Lab accession number: pRM1665
Proper citation: RRID:Addgene_154887 Copy
Species: Homo sapiens
Genetic Insert: KDM4D, GLIS1
Vector Backbone Description: Vector Backbone:pCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33798453
Proper citation: RRID:Addgene_154880 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Addgene, #73638; Backbone Size:5211; Vector Backbone:pDEST-ORF-V2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33420373
Proper citation: RRID:Addgene_154895 Copy
Species: Homo sapiens
Genetic Insert: ERBB3
Vector Backbone Description: Backbone Marker:Addgene, #73637; Backbone Size:5439; Vector Backbone:pDEST-ORF-v1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33420373
Proper citation: RRID:Addgene_154893 Copy
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