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Species: Homo sapiens
Genetic Insert: HOXC6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3-HISA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15637592
Proper citation: RRID:Addgene_110365 Copy
Species: Homo sapiens
Genetic Insert: HOXC6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3-HISA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15637592
Proper citation: RRID:Addgene_110366 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6278; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_110401 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:#50005; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Homology arm for C-Terminal knock-in at stop codon of GLS' KGA isoform. Just mKO2 knock-in.
Construction order:
GLS 1kb 5' Homology Arm: SacI-BamHI;
GLS 1kb 3' Homology Arm BamHI-SalI;
BglII-BamHI mKO2
System options:
sgRNA:
pX330.puro sgRNA KGA Stop1 (Addgene #110405)
Homology arms:
pUC19 KGA HA5'-mKO2-3' (Addgene #110406)
pUC19 KGA HA5'-mKO2-P2A-3' (Addgene #110407)
pUC19 KGA HA5'-mKO2-P2A-Zeo-3' (Addgene #110408)
Proper citation: RRID:Addgene_110406 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:#110403; Backbone Size:9906; Vector Backbone:pX330.puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: sgRNA for GLS exon 19 (KGA isoform stop codon), intended to perform C-Terminal knock-in using pX330 with puromycin resistance.
System options:
sgRNA:
pX330.puro sgRNA KGA Stop1 (Addgene #110405)
Homology arms:
pUC19 KGA HA5'-mKO2-3' (Addgene #110406)
pUC19 KGA HA5'-mKO2-P2A-3' (Addgene #110407)
pUC19 KGA HA5'-mKO2-P2A-Zeo-3' (Addgene #110408)
Proper citation: RRID:Addgene_110405 Copy
Species: Homo sapiens
Genetic Insert: PKCZ
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110512 Copy
Species: Homo sapiens
Genetic Insert: PLEKHS1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110510 Copy
Species: Homo sapiens
Genetic Insert: KGA glutaminase
Vector Backbone Description: Backbone Marker:#1766; Backbone Size:4902; Vector Backbone:pBABE-zeo (pBABE-bleo); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110357 Copy
Species: Homo sapiens
Genetic Insert: NDP52
Vector Backbone Description: Backbone Size:5747; Vector Backbone:pBMN-mEGFP-C1; Vector Types:Mammalian Expression, Retroviral, retrovirus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26266977
Proper citation: RRID:Addgene_110465 Copy
Species: Homo sapiens
Genetic Insert: ELAV like RNA binding protein 1
Vector Backbone Description: Backbone Marker:Plasmid #1764; Backbone Size:5177; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110346 Copy
Species: Homo sapiens
Genetic Insert: Rab13
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22674975
Proper citation: RRID:Addgene_110500 Copy
Species: Homo sapiens
Genetic Insert: CCDC32
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110505 Copy
Species: Homo sapiens
Genetic Insert: Rab5C-S35N
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22674975
Proper citation: RRID:Addgene_110504 Copy
Species: Homo sapiens
Genetic Insert: PLEKHS1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110509 Copy
Species: Homo sapiens
Genetic Insert: IQCF2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110507 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:7583; Vector Backbone:pCMV histidinol; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10958681
Comments: pCMV human IRS-1 was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into pCMV human IRS-1. Select cells expressing plasmid with approximately 5mM histidinol (Sigma), depending on the cell type.
Proper citation: RRID:Addgene_11238 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10958681
Comments: IRS-1 pBABE was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into IRS-I pBABE.
(Kahn lab plasmid name = IRS-1 CAAX (Human) pBABE)
Proper citation: RRID:Addgene_11237 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Proper citation: RRID:Addgene_11232 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Proper citation: RRID:Addgene_11231 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Proper citation: RRID:Addgene_11234 Copy
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