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Species: Other
Genetic Insert: Para - lp-DsRed-parB
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Proper citation: RRID:Addgene_128303 Copy
Species: Other
Genetic Insert: Para - DsRed-parB
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Proper citation: RRID:Addgene_128302 Copy
Species: Other
Genetic Insert: Para - GCN4zip.MSE-parB
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Proper citation: RRID:Addgene_128300 Copy
Species: Other
Genetic Insert: AsCas12a-Triplex
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31406383
Proper citation: RRID:Addgene_128405 Copy
Species: Other
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Ekker Lab; Backbone Size:4651; Vector Backbone:pT3TS-delta-TAL; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31366908
Comments: Plasmid contains a lot of repetitive regions and thus next generation sequencing had difficulty assembling a full plasmid sequence. Due to this Addgene's full plasmid sequence contains several ambiguous bases and a few mismatches in non-essential regions compared to depositor sequence. Depositor confirms plasmid is functional!
Proper citation: RRID:Addgene_128693 Copy
Species: Other
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Ekker Lab; Backbone Size:4651; Vector Backbone:pT3TS-delta-TAL; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31366908
Comments: Plasmid contains a lot of repetitive regions and thus next generation sequencing had difficulty assembling a full plasmid sequence. Due to this Addgene's full plasmid sequence contains several ambiguous bases and a few mismatches in non-essential regions compared to depositor sequence. Depositor confirms plasmid is functional!
Proper citation: RRID:Addgene_128696 Copy
Species: Other
Genetic Insert: NanoLuc
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:4762; Vector Backbone:pDONR-221; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31366908
Proper citation: RRID:Addgene_128673 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:8316; Vector Backbone:pLVX-GWB-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128660 Copy
Species: Other
Genetic Insert: Niben101Scf04395g00017.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_126379 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Derived in Kotton lab; Backbone Size:7836; Vector Backbone:p1303 DV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28965766
Proper citation: RRID:Addgene_126696 Copy
Species: Other
Genetic Insert: human SPC right
Vector Backbone Description: Backbone Marker:Derived in Kotton lab; Backbone Size:6413; Vector Backbone:EF1a-TALEN; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28965766
Comments: Please note that the Addgene verified sequence finds an R451P mutation in SPC and a deletion of the 0.5 repeat HD region when compared to the depositor's sequence. These differences do not impact plasmid function.
Proper citation: RRID:Addgene_126695 Copy
Species: Other
Genetic Insert: filamin-mEos3.2
Vector Backbone Description: Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126808 Copy
Species: Other
Genetic Insert: mEos3.2
Vector Backbone Description: Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126806 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pSMART-HC-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32441815
Comments: Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_127058 Copy
Species: Other
Genetic Insert: uORF-Cas9
Vector Backbone Description: Backbone Marker:Johannes Bischof and Konrad Basler; Backbone Size:8500; Vector Backbone:pUASTattB; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32053108
Proper citation: RRID:Addgene_127382 Copy
Species: Other
Genetic Insert: sgRNA frame 1
Vector Backbone Description: Backbone Marker:Fillip Port and Simon Bullock; Vector Backbone:pCFD3; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31685521
Proper citation: RRID:Addgene_127554 Copy
Species: Other
Genetic Insert: Integrase phiC31 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pBluescript II SK(-); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).
Proper citation: RRID:Addgene_127535 Copy
Species: Other
Genetic Insert: Integrase 13 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 13 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127534 Copy
Species: Other
Genetic Insert: Integrase 2 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 2 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127529 Copy
Species: Other
Genetic Insert: Integrase 7 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 7 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014). The related two SNPs mutations were due to the synthesis building process. The resulted gene coding sequence was the only one possible to be obtained by the synthesis company.
Proper citation: RRID:Addgene_127515 Copy
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