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Species: Saccharomyces cerevisiae
Genetic Insert: SRP14
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253000 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: COG5
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253002 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC4
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253006 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252966 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252968 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252969 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: JEM1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253011 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pir
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252962 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Aga2 Anti-GFP nanobody
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253042 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Suc2
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253022 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253025 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253026 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253028 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253030 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Anti-GFP nanobody -Aga2
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253031 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Anti-GFP nanobody -Aga1
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253035 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABE8e
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Proper citation: RRID:Addgene_251975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABE8e
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41543903
Comments: Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.
Proper citation: RRID:Addgene_251974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Leaf compost cutinase
Vector Backbone Description: Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253049 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NcoI_SMT3-SUMO(21-98)_C_sTEV_His6
Vector Backbone Description: Backbone Size:5237; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41927759
Comments: This vector is a modified pET29b(+) vector that includes one added cloning site (NcoI) followed by the encoding region for a fragment of the SMT3_yeast gene (21-98) followed by a TEV site (ENLYFQS) and a hexa-histidine site (HHHHHH*). Any peptide of interest can be cloned in the vector using the two cloning sites (NdeI and NcoI) via HiFi assembly. When adding a peptide of interest this way, the remaining N-terminal portion of the SMT3 gene should be added as well, separated by the peptide of interest with a GSGS encoding region (GSGSMSDSEVNQEAKPEVKPEVKP). This can result in a vector which encodes for the expression of a recombinant gene of the following type : PEPTIDE-GSGS-SUMO(1-98)-C-sTEV_His6*.
Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint.
Proper citation: RRID:Addgene_254142 Copy
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