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Species: Other
Genetic Insert: HCoV-OC43-nsp4
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168932 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp5
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168933 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp6
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168934 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp9
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168937 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp10
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168938 Copy
Species: Other
Genetic Insert: HCoV-NL63-Protein3
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168923 Copy
Species: Other
Genetic Insert: HCoV-NL63-Envelope
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Please note that Addgene identified an IS4-like element transposase in the plasmid backbone. This should not affect Gateway cloning experiments since it is outside of the Gateway cassette.
Proper citation: RRID:Addgene_168924 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp12
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168927 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:2651; Vector Backbone:PAM24; Vector Types:Acanthamoeba expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37964008
Proper citation: RRID:Addgene_198744 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:5667; Vector Backbone:PAM3; Vector Types:Acanthamoeba expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37964008
Proper citation: RRID:Addgene_198742 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:5427; Vector Backbone:PAM1; Vector Types:Acanthamoeba expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37964008
Proper citation: RRID:Addgene_198740 Copy
Species: Other
Genetic Insert: H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Recombinant baculovirus production (Bac-to-Bac); Bacterial Resistance:Gentamicin and Spectinomycin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206252 Copy
Species: Other
Genetic Insert: PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV PE2; Vector Types:Mammalian Expression, CRISPR, Recombinant baculovirus production, CRE-acceptor; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206274 Copy
Species: Other
Genetic Insert: PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV PE2; Vector Types:Mammalian Expression, CRISPR, Recombinant baculovirus production; Bacterial Resistance:Chloramphenicol and Gentamicin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206273 Copy
Species: Other
Genetic Insert: ISCytsp1 TnpB
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37386294
Proper citation: RRID:Addgene_205339 Copy
Species: Other
Genetic Insert: ISCvi2 TnpB
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37386294
Proper citation: RRID:Addgene_205337 Copy
Species: Other
Genetic Insert: ISMmu1 TnpB
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37386294
Proper citation: RRID:Addgene_205349 Copy
Species: Other
Genetic Insert: ISMac7 TnpB
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37386294
Proper citation: RRID:Addgene_205347 Copy
Species: Other
Genetic Insert: ISKpn69 TnpB
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37386294
Proper citation: RRID:Addgene_205346 Copy
Species: Other
Genetic Insert: ISEfa4 TnpB
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37386294
Proper citation: RRID:Addgene_205344 Copy
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