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pYSD515
 
Resource Report
Resource Website 		RRID:Addgene_253000 SRP14 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD517
 
Resource Report
Resource Website 		RRID:Addgene_253002 COG5 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD521
 
Resource Report
Resource Website 		RRID:Addgene_253006 SEC4 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD053
 
Resource Report
Resource Website 		RRID:Addgene_252966 Aga2 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD055
 
Resource Report
Resource Website 		RRID:Addgene_252968 Aga2 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD056
 
Resource Report
Resource Website 		RRID:Addgene_252969 Aga2 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD526
 
Resource Report
Resource Website 		RRID:Addgene_253011 JEM1 Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:41 0
pYSD046
 
Resource Report
Resource Website 		RRID:Addgene_252962 Pir Saccharomyces cerevisiae Chloramphenicol PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:35:40 0
pYSD332
 
Resource Report
Resource Website 		RRID:Addgene_253042 Aga2 Anti-GFP nanobody Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD281
 
Resource Report
Resource Website 		RRID:Addgene_253022 Suc2 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD292
 
Resource Report
Resource Website 		RRID:Addgene_253025 AGA1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD293
 
Resource Report
Resource Website 		RRID:Addgene_253026 AGA1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD295
 
Resource Report
Resource Website 		RRID:Addgene_253028 AGA1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD299
 
Resource Report
Resource Website 		RRID:Addgene_253030 AGA1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD301
 
Resource Report
Resource Website 		RRID:Addgene_253031 Anti-GFP nanobody -Aga2 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:John Dueber, Addgene plasmid #65190; Vector Backbone:pYTK083; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
pYSD316
 
Resource Report
Resource Website 		RRID:Addgene_253035 Anti-GFP nanobody -Aga1 Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:35:41 0
MTS-ABE8e(∆REC2)-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_251975 ABE8e Saccharomyces cerevisiae Ampicillin PMID:41543903 Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint. Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin REC2 deleted 2026-08-15 01:35:45 0
MTS-ABE8e(∆HNH)-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_251974 ABE8e Saccharomyces cerevisiae Ampicillin PMID:41543903 Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint. Vector Backbone:pAG415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin HNH deleted 2026-08-15 01:35:45 0
pYSD726
 
Resource Report
Resource Website 		RRID:Addgene_253049 Leaf compost cutinase Saccharomyces cerevisiae Ampicillin PMID:42025218 YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint. Backbone Marker:Paul Young, Addgene plasmid #212923; Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Y127G, D238C, F243I, S283C 2026-08-15 01:35:45 0
pET29b(+)_NdeI-NcoI_SUMO(21-98)_CV
 
Resource Report
Resource Website 		RRID:Addgene_254142 NcoI_SMT3-SUMO(21-98)_C_sTEV_His6 Saccharomyces cerevisiae Kanamycin PMID:41927759 This vector is a modified pET29b(+) vector that includes one added cloning site (NcoI) followed by the encoding region for a fragment of the SMT3_yeast gene (21-98) followed by a TEV site (ENLYFQS) and a hexa-histidine site (HHHHHH*). Any peptide of interest can be cloned in the vector using the two cloning sites (NdeI and NcoI) via HiFi assembly. When adding a peptide of interest this way, the remaining N-terminal portion of the SMT3 gene should be added as well, separated by the peptide of interest with a GSGS encoding region (GSGSMSDSEVNQEAKPEVKPEVKP). This can result in a vector which encodes for the expression of a recombinant gene of the following type : PEPTIDE-GSGS-SUMO(1-98)-C-sTEV_His6*. Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint. Backbone Size:5237; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Contains encoding region for enzyme site NdeI, then followed by the 21-98 AA of SMT3_yeast, followed by encoding region for a Cystein, a TEV site and a hexa-histidine encoding region. 2026-08-15 01:35:46 0

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