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Species: Danio rerio
Genetic Insert: antiCD19-Notch3-GL4VP64
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32002793
Proper citation: RRID:Addgene_169916 Copy
Species: Mus musculus
Genetic Insert: Grin1
Vector Backbone Description: Vector Backbone:gRNA backbone; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161760
Proper citation: RRID:Addgene_169789 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-5A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8275867
Comments: Insert: cDNA from Melton lambda gt10 oocyte library.
Transcription: linearize with Xba1 and transcribe with SP6.
Plasmid History:
Constructed by Randall Moon on date of 9/15/90.
Notes for Use:
EcoR1 insert of lambda G6 of 2/11/90. Contains significant 5' UTR and is less translatable in vitro per ng RNA than Xwnt-5A Hind III/SP64T. However, the type of activity is the same in both constructs.
Citation: Moon et al., Development 119, 97 (1993)
Proper citation: RRID:Addgene_16985 Copy
Species: Xenopus laevis
Genetic Insert: banded hedgehog
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Xenopus Hedgehog. Antisense are generated as listed:
Xenopus banded hedgehog: Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus sonic hedgehog: Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus cephalic hedgehog:Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7.
Proper citation: RRID:Addgene_16984 Copy
Vector Backbone Description: Backbone Size:4663; Vector Backbone:pEPIC1.1; Vector Types:Mammalian Expression, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917762
Proper citation: RRID:Addgene_169897 Copy
Vector Backbone Description: Backbone Size:2427; Vector Backbone:pPID2; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917762
Proper citation: RRID:Addgene_169898 Copy
Species: Homo sapiens
Genetic Insert: UDP-N-acetylhexosamine pyrophosphorylase
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pSin4 EF1a IRES Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34288588
Comments: This plasmid was generated in the lab a long time ago and we cannot locate detailed cloning information at this point.
Proper citation: RRID:Addgene_169891 Copy
Species: Synthetic
Genetic Insert: sgRNA targeting ACO1
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:11590; Vector Backbone:pLENTICRISPR V2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34039609
Comments: Target: CCACTACCCCAACCTGGCGG
Proper citation: RRID:Addgene_169892 Copy
Species: Homo sapiens
Genetic Insert: Vangl2/Strabismus
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: This is a wild type human VANGL2/Strabismuus, cloned into pCS2P+. Injected RNA in zebrafish embryos gives the expected convergent/extension phenotype (Chris Thorpe, unpub. observations).
Reference: Made by Yan Zhang by PCR for RTM. Cloned as a Cla1-Xho1 insert.
Proper citation: RRID:Addgene_16992 Copy
Species: Synthetic
Genetic Insert: sgRNA targeting IREB2
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:11590; Vector Backbone:pLENTICRISPR V2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34039609
Comments: Target: AATGCACCAAATCCTGGAGG
Proper citation: RRID:Addgene_169894 Copy
Species: Homo sapiens
Genetic Insert: TFRC
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pMXS-IRES-BLAST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34039609
Proper citation: RRID:Addgene_169890 Copy
Species: Homo sapiens
Genetic Insert: tCD19
Vector Backbone Description: Vector Backbone:PX458; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33903218
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.22.427783v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_169817 Copy
Species: Mus musculus
Genetic Insert: pegCTNNB1 S45DEL
Vector Backbone Description: Vector Backbone:pmd127; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169844 Copy
Species: Mus musculus
Genetic Insert: sgCTNNB1 Nicking
Vector Backbone Description: Vector Backbone:pmd127; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169845 Copy
Species: Homo sapiens
Genetic Insert: sgAAT Nicking
Vector Backbone Description: Vector Backbone:pmd127; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169842 Copy
Species: SARS coronavirus 2
Genetic Insert: Protein S
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10381; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35046611
Proper citation: RRID:Addgene_169848 Copy
Species: SARS coronavirus 2
Genetic Insert: Protein S
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10381; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35046611
Proper citation: RRID:Addgene_169849 Copy
Species: Xenopus laevis
Genetic Insert: Goosecoid
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian by PCR.
Goosecoid cloned and characterized by lab of E. DeRobertis, UCLA.
Proper citation: RRID:Addgene_16979 Copy
Species: Mus musculus
Genetic Insert: safe harbor sgRNA
Vector Backbone Description: Backbone Size:8907; Vector Backbone:pMCB320; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157015
Proper citation: RRID:Addgene_169834 Copy
Species: Xenopus laevis
Genetic Insert: Xbra
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:psp73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: full length Xbra from Jim Smith.
Antisense: linearize with EcoRV, transcribe with T7.
Plasmid History:
Constructed by Jan Christian.
Proper citation: RRID:Addgene_16978 Copy
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