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Species: Other
Genetic Insert: Integrase 2 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 2 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127512 Copy
Species: Other
Genetic Insert: Integrase Bxb1 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pBluescript II SK(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: Please note: In 2987 plasmid position, in the UbC promoter, there is a SNP where a G was changed for a T.
The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The UbC promoter and _-globin poly(A) signal sequences are derived from pUB-GFP plasmid (Addgene #11155). The original Bxb1 coding sequence (before H. sapiens codon optimization) was obtained from Bonnet, J. et al. Science 340, 599-603 (2013) (Genbank KC529324.1).
Proper citation: RRID:Addgene_127519 Copy
Species: Other
Genetic Insert: Integrase 9 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 9 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127516 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pSin; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127695 Copy
Species: Other
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:Thermo scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Golden gate donor vector for gene targeting in Bacillus subtilis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31147909
Comments: Donor vector that encodes for a C-terminal Superfolder GFP tag for Golden gate assembly reactions. Overhangs generated upon BsaI cleavage : AGCG (RtagN) and TAAG (RtagC).
(Gruber lab reference pSG710)
Proper citation: RRID:Addgene_117123 Copy
Species: Other
Genetic Insert: ZmMATE2
Vector Backbone Description: Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_117094 Copy
Species: Other
Genetic Insert: AtTPT (ORF-HIS)
Vector Backbone Description: Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: An A234T difference in AtTPT compared to the reference sequence is present but does not impact the function of the plasmid.
Proper citation: RRID:Addgene_117093 Copy
Species: Other
Genetic Insert: shRNA targeting miR30
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_117158 Copy
Species: Other
Genetic Insert: shRNA targeting miR30
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_117159 Copy
Species: Other
Genetic Insert: shRNA targeting miR30
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_117161 Copy
Species: Other
Genetic Insert: shRNA targeting miR30
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_117160 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117231 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117232 Copy
Species: Other
Genetic Insert: G-CaMP3
Vector Backbone Description: Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29939997
Proper citation: RRID:Addgene_117422 Copy
Species: Other
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29939997
Proper citation: RRID:Addgene_117421 Copy
Species: Other
Genetic Insert: G-CaMP6s
Vector Backbone Description: Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29939997
Proper citation: RRID:Addgene_117423 Copy
Species: Other
Genetic Insert: pre-pro-alphaf-Btl2
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:30314480
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117664 Copy
Species: Other
Genetic Insert: Htb2-GFP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30314480
Comments: Integration plasmid in the pGAP locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_117669 Copy
Species: Other
Genetic Insert: pre-pro-alphaf-E2-Crimson
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:30314480
Comments: Integration plasmid in the pAOX1 locus.
Proper citation: RRID:Addgene_117658 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:pLX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117730 Copy
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