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Species: SARS-Cov2
Genetic Insert: SARS-Cov2 spike_deleted
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Generation of SARS-CoV-2-pseudotyped lentiviral particles; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34547465
Proper citation: RRID:Addgene_155297 Copy
Species: Synthetic
Genetic Insert: iRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155229 Copy
Species: Homo sapiens
Genetic Insert: mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155227 Copy
Species: Homo sapiens
Genetic Insert: Membrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
Vector Backbone Description: Vector Backbone:pCAGENB HYJ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155228 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155221 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155222 Copy
Vector Backbone Description: Backbone Size:9512; Vector Backbone:Addgene #155182; Vector Types:Mammalian Expression, Unspecified, Piggybac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35567999
Comments: The T2A-Puro sequence was re-amplified and cloned into the backbone to remove BbsI restriction site in the T2A sequence. To clone Cas13d gRNA for gene knockdown, digest the plasmid with BbsI and then ligate annealed oligo into the backbone. gRNA could be designed using https://cas13design.nygenome.org/ and annealing oligoes should have the following format, where Ns and Ms are the gRNA sequence and its reverse-complement counterpart:
FWD:5'- AAACNNNNNNNNNNNNN
RVS: 5'-AAAAMMMMMMMMMMM
Proper citation: RRID:Addgene_155184 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155220 Copy
Species: Mus musculus
Genetic Insert: Wdr5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15553 Copy
Species: Mus musculus
Genetic Insert: Wdr5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15552 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15551 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15550 Copy
Species: Homo sapiens
Genetic Insert: FKBP12(V36)-p30Caspase9
Vector Backbone Description: Backbone Size:5300; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728125
Comments: Based on L. Fan et al (99) Human Gene Therapy
Proper citation: RRID:Addgene_15567 Copy
Species: Synthetic
Genetic Insert: H11-H4
Vector Backbone Description: Vector Backbone:pOPINO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32661423
Proper citation: RRID:Addgene_155364 Copy
Species: Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens
Genetic Insert: dCas13-M3nls
Vector Backbone Description: Vector Backbone:pCMV with pBR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32601430
Proper citation: RRID:Addgene_155366 Copy
Species: Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens
Genetic Insert: dCas13-M3M14nes
Vector Backbone Description: Vector Backbone:pCMV with pBR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32601430
Proper citation: RRID:Addgene_155367 Copy
Species: Homo sapiens
Genetic Insert: FOXC2
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17537911
Proper citation: RRID:Addgene_15535 Copy
Species: Homo sapiens
Genetic Insert: Ash2L
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Comments: Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'.
Proper citation: RRID:Addgene_15549 Copy
Species: Homo sapiens
Genetic Insert: Ash2L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Comments: Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'
Please note that mutation R622H in ASH2L was found during Addgene's quality control. The plasmid should function as described.
Proper citation: RRID:Addgene_15548 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 2, VI in article.
Lindquist lab number: 7704
Proper citation: RRID:Addgene_15579 Copy
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