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Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:9879; Vector Backbone:pLX311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30777879
Proper citation: RRID:Addgene_117731 Copy
Species: Other
Genetic Insert: NanoLuc
Vector Backbone Description: Backbone Size:5097; Vector Backbone:pNBU2-ErmG; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28431252
Proper citation: RRID:Addgene_117727 Copy
Species: Other
Genetic Insert: YlpTEF_hCas9_YlCyc1TT
Vector Backbone Description: Backbone Size:4193; Vector Backbone:pMEG_BB3_YL68N_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Nourseothricin (clonNat)
Defining Citation: PMID:30698703
Proper citation: RRID:Addgene_117826 Copy
Species: Other
Genetic Insert: YlMig1TT
Vector Backbone Description: Backbone Size:1966; Vector Backbone:BB1_L_34_syn_BsaI; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30698703
Proper citation: RRID:Addgene_117822 Copy
Species: Other
Genetic Insert: YlCyc1TT
Vector Backbone Description: Backbone Size:2028; Vector Backbone:pIDTSMART-KAN; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30698703
Proper citation: RRID:Addgene_117821 Copy
Species: Other
Genetic Insert: YlpTEF_YlGUT1_YlCyc1TT
Vector Backbone Description: Backbone Size:2215; Vector Backbone:BB2_L_AB_syn_BbsI; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30698703
Proper citation: RRID:Addgene_117824 Copy
Species: Other
Genetic Insert: MjaTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118396 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118398 Copy
Species: Other
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pDONR-P2R-P3z; Vector Types:Unspecified; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32601420
Comments: Addgene NGS identified an in-frame insertion in the ccdB gene in this plasmid, the depositing lab confirmed that this variant was present in their initial stock of the plasmid and has functioned as expected.
Proper citation: RRID:Addgene_118389 Copy
Species: Other
Genetic Insert: AtMIR390a backbone
Vector Backbone Description: Vector Backbone:pDONR-221z; Vector Types:RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32601420
Proper citation: RRID:Addgene_118388 Copy
Species: Other
Genetic Insert: dCAS9p
Vector Backbone Description: Vector Backbone:pDONR-221z; Vector Types:CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32601420
Proper citation: RRID:Addgene_118387 Copy
Species: Other
Genetic Insert: AtU3b promoter
Vector Backbone Description: Vector Backbone:pDONR-P2R-P3z; Vector Types:CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32601420
Proper citation: RRID:Addgene_118390 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118403 Copy
Species: Other
Genetic Insert: MjaTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118402 Copy
Species: Other
Genetic Insert: sgRNA/Puro-T2A-BFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30318302
Proper citation: RRID:Addgene_118594 Copy
Species: Other
Genetic Insert: HA-dCas9
Vector Backbone Description: Backbone Size:11095; Vector Backbone:pAT1502; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134895 Copy
Species: Other
Genetic Insert: GFP-V5-miniTurbo
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135240 Copy
Species: Other
Genetic Insert: GFP-V5-TurboID
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135241 Copy
Species: Other
Genetic Insert: TurboID-V5-dsRed
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135261 Copy
Species: Other
Genetic Insert: V5-dsRed
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135262 Copy
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