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Species: Homo sapiens
Genetic Insert: guide RNA for KLF7 neighbouring region
Vector Backbone Description: Backbone Marker:Addgene Plasmid #52961; Backbone Size:12993; Vector Backbone:LentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30733539
Proper citation: RRID:Addgene_169797 Copy
Species: Homo sapiens
Genetic Insert: Donor sequence to ACTB gene for HR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2959; Vector Backbone:pBlueScript II SK (+); Vector Types:HR donor vector for human ACTB gene.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30733539
Proper citation: RRID:Addgene_169798 Copy
Species: Synthetic
Genetic Insert: OxLight1
Vector Backbone Description: Backbone Marker:Viral Vector Facility - University of Zurich; Backbone Size:4438; Vector Backbone:pAAV.hSynapsin1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35145320
Proper citation: RRID:Addgene_169792 Copy
Genetic Insert: Scrambled guide RNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #52961; Backbone Size:12993; Vector Backbone:LentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30733539
Proper citation: RRID:Addgene_169795 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:8535; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Proper citation: RRID:Addgene_169745 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5422; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Proper citation: RRID:Addgene_169746 Copy
Vector Backbone Description: Backbone Size:6529; Vector Backbone:pPIDCB2; Vector Types:Mammalian Expression, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917762
Comments: Use AflII and PstI restriction sites for cloning. This plasmid is identical to pPIDNB2 except it has replaced the mNeonGreen sequence in pPIDNB2, which requires a paid Allele Biotech license, with mClover3, which doesn't require a paid license.
Proper citation: RRID:Addgene_169900 Copy
Species: Synthetic
Genetic Insert: tetR-P2A-eGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6113; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169747 Copy
Vector Backbone Description: Backbone Size:6916; Vector Backbone:pPIDNB2; Vector Types:Mammalian Expression, Avian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917762
Comments: Use AflII and PstI restriction sites for cloning. This plasmid contains mNeonGreen, which requires a paid license. See pPIDCB2 for an alternative plasmid, which has replaced the mNeongreen with mClover3 and does not require a paid license.
Proper citation: RRID:Addgene_169902 Copy
Genetic Insert: SauCas9 EMX1-nicking sgRNA
Vector Backbone Description: Backbone Size:600; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169860 Copy
Genetic Insert: Spy EMX1-pegRNA
Vector Backbone Description: Backbone Size:600; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169855 Copy
Genetic Insert: SaCas9KKH-N580A-M-MLV
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169852 Copy
Genetic Insert: SpCas9-H840A-star-M-MLV-3HA
Vector Backbone Description: Backbone Size:6635; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169854 Copy
Genetic Insert: spCas9 PE*
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 U57A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Comments: Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.
Proper citation: RRID:Addgene_169927 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 U57C
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Comments: Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.
Proper citation: RRID:Addgene_169928 Copy
Species: Homo sapiens
Genetic Insert: kinesin family member 5B, isoform CRA_a
Vector Backbone Description: Backbone Marker:Novagene; Backbone Size:3306; Vector Backbone:pET-17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30770448
Proper citation: RRID:Addgene_169995 Copy
Species: Homo sapiens
Genetic Insert: Kinesin-like protein Unc-104 fused with KIF5B
Vector Backbone Description: Backbone Marker:Novagene; Backbone Size:3306; Vector Backbone:pET-17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203385
Proper citation: RRID:Addgene_169996 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).
Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170019 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169940 Copy
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