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Species: Mus musculus
Genetic Insert: Krt75-ex5
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59244 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Marker:Addgene #10878; Backbone Size:7032; Vector Backbone:pLKO.1-TRC cloning vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: TRC Clone ID TRCN0000217421
Proper citation: RRID:Addgene_70058 Copy
Species: Mus musculus
Genetic Insert: eIF4A1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV IRES GFP (MIG); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25079319
Proper citation: RRID:Addgene_70043 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Size:11522; Vector Backbone:CS-TRE-mOKS-PRE-Ubc-rTTA-I2G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: Silent mutations are introduced to escape from RNAi using pLKO.1-shDnd1-No. 1,6, and 8 deposited in Addgene (plasmids #70058, 70067, and 70069).
Proper citation: RRID:Addgene_70076 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Marker:Addgene #10878; Backbone Size:7032; Vector Backbone:pLKO.1-TRC cloning vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: TRC Clone ID TRCN0000247050
Proper citation: RRID:Addgene_70068 Copy
Species: Mus musculus
Genetic Insert: Dnd1
Vector Backbone Description: Backbone Marker:Addgene #10878; Backbone Size:7032; Vector Backbone:pLKO.1-TRC cloning vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28297718
Comments: TRC Clone ID TRCN0000247051
Proper citation: RRID:Addgene_70067 Copy
Species: Mus musculus
Genetic Insert: Eomes
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.); Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments:
The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_70271 Copy
Species: Mus musculus
Genetic Insert: GATA binding protein 3
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.) ; Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The coding sequence for murine Gata3 (together with parts of the 5' and 3' UTR) was inserted into the EcoRI site of the pLV-tetO vector (pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, Addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University)
Proper citation: RRID:Addgene_70270 Copy
Species: Mus musculus
Genetic Insert: Ets2
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.) ; Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The cDNA sequence of murine Ets2 was amplified from undifferentiated trophoblast stem cells and inserted into the pCR2.1 vector. From there it was excised by EcoRI digest and ligated into the pLV-tetO vector via the EcoRI site. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_70272 Copy
Species: Mus musculus
Genetic Insert: Tfap2a
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.) ; Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The cDNA sequence of murine Tfap2a (coding region and partially 5' and 3' UTR) was inserted into the pLV-tetO vector via the EcoRI site. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_70274 Copy
Species: Mus musculus
Genetic Insert: Trophoblast specific protein alpha
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.); Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The coding sequence for murine Tpbpa (together with parts of the 5' and 3' UTR) was inserted into the EcoRI site of the pLV-tetO vector (pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, Addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University)
Proper citation: RRID:Addgene_70698 Copy
Species: Mus musculus
Genetic Insert: 5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´
Vector Backbone Description: Vector Backbone:pBluescript/U6; Vector Types:Mammalian Expression, U6 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21788450
Comments: Please note there is a single nucleotide mismatch between the depositor's sequence and Addgene's quality control sequence. This mismatch does not affect plasmid function.
Proper citation: RRID:Addgene_70666 Copy
Species: Mus musculus
Genetic Insert: Tropomodulin3 1-163 AA fragment
Vector Backbone Description: Backbone Size:5006; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25575350
Proper citation: RRID:Addgene_70754 Copy
Species: Mus musculus
Genetic Insert: Elf5
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.); Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The cDNA sequence of murine Elf5 was amplified from trophoblast stem cell cDNA and subcloned into the pCR2.1 vector. From there it was excised via BamHI and EcoRV and inserted into the pLV-tetO vector via the EcoRI site after Klenow treatment of both vector backbone and insert. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_70772 Copy
Species: Mus musculus
Genetic Insert: ATP Citrate Lyase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pEF6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24998913
Proper citation: RRID:Addgene_70765 Copy
Species: Mus musculus
Genetic Insert: inhibitor of DNA binding 2
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.); Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The cDNA sequence of murine Id2 was amplified and subcloned into the pCR2.1 vector. From there it was excised via HindIII and EcoRV and inserted into the pLV-tetO vector via the EcoRI site after Klenow treatment of both vector backbone and insert. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_70764 Copy
Species: Mus musculus
Genetic Insert: Grp1-PH
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9804818
Proper citation: RRID:Addgene_71378 Copy
Species: Mus musculus
Genetic Insert: Grp1-PH
Vector Backbone Description: Backbone Marker:Amersham-Pharmacia; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9804818
Proper citation: RRID:Addgene_71379 Copy
Species: Mus musculus
Genetic Insert: Grp1-PH
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10983984
Proper citation: RRID:Addgene_71377 Copy
Species: Mus musculus
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24469447
Proper citation: RRID:Addgene_71366 Copy
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