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Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 1C in article.
Lindquist lab number: 7720
Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.
Proper citation: RRID:Addgene_15587 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832050
Comments: Figure 1C in article.
Lindquist lab number: 7719
Proper citation: RRID:Addgene_15584 Copy
Species: Homo sapiens
Genetic Insert: YTHDF1
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155346 Copy
Species: Homo sapiens
Genetic Insert: YTHDF2
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155347 Copy
Species: Synthetic
Genetic Insert: humanized EBpaRS/BstYam
Vector Backbone Description: Backbone Marker:Clontech, major modifications; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929862
Comments: The plasmid has been developed for efficient incorporation of Bpa in mammalian cells, first reported in Böttke et al, EMBO reports, 2020. With respect to similar plasmids from other labs, this plasmid contains a humanized gene for EBpaRS and 4 copies of the U6-BstYam tRNA cassette downstream of the synthetase cassette.
Proper citation: RRID:Addgene_155342 Copy
Species: Homo sapiens
Genetic Insert: IKB alpha
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17574021
Proper citation: RRID:Addgene_15264 Copy
Species: Mus musculus
Genetic Insert: Neuroligin 1 with insert B
Vector Backbone Description: Backbone Size:4921; Vector Backbone:pCAAGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16846852
Proper citation: RRID:Addgene_15261 Copy
Species: Mus musculus
Genetic Insert: Neuroligin 1
Vector Backbone Description: Backbone Size:4921; Vector Backbone:pCAAGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16846852
Comments: There is a A43V mutation in the aa acid sequence. The mutation is within the signal sequence and our data demonstrate that cleavage is normal. That means the mature protein does not have any change in amino acid sequence.
Proper citation: RRID:Addgene_15260 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 N (nucleocapsid)
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724397.2
Proper citation: RRID:Addgene_152594 Copy
Species: Homo sapiens
Genetic Insert: BRAF
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17574021
Proper citation: RRID:Addgene_15269 Copy
Species: Homo sapiens
Genetic Insert: inhibitor of Kappa B alpha
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17574021
Proper citation: RRID:Addgene_15290 Copy
Species: Homo sapiens
Genetic Insert: RARS
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Vector Backbone:pNIC-Bio3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Backbone vector pNIC-Bio3, Genbank acc. no. JN792439
Proper citation: RRID:Addgene_153055 Copy
Species: Homo sapiens
Genetic Insert: BHLHE40
Vector Backbone Description: Backbone Size:8871; Vector Backbone:pLVX-EF1α-IRES-ZsGreen1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32205878
Proper citation: RRID:Addgene_153061 Copy
Genetic Insert: VP16AD-ZIP+
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pActPL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17088209
Comments: The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The EE12RR345L (Zip+) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.
Proper citation: RRID:Addgene_15305 Copy
Genetic Insert: Gal4AD-ZIP+
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pActPL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17088209
Comments: The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The EE12RR345L (Zip+) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.
Proper citation: RRID:Addgene_15303 Copy
Genetic Insert: sTurboID (C) (G74)-ERM (Cb5)
Vector Backbone Description: Backbone Size:8233; Vector Backbone:pLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32424107
Proper citation: RRID:Addgene_153007 Copy
Genetic Insert: sTurboID (N) (L73)
Vector Backbone Description: Backbone Size:7668; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32424107
Proper citation: RRID:Addgene_153002 Copy
Species: Synthetic
Genetic Insert: ABE7.10-nSpCas9
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152989 Copy
Species: Synthetic
Genetic Insert: eA3A-nSpCas9 NG-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152999 Copy
Species: Synthetic
Genetic Insert: eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152998 Copy
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