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Species: Homo sapiens
Genetic Insert: mScarlet-F-hgp100-T2A-BlastR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2577; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171815 Copy
Species: Discosoma sp., Bacillus cereus
Genetic Insert: mScarlet-T2A-BlastR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2577; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171814 Copy
Species: Synthetic
Genetic Insert: DogCatcher
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171772 Copy
Species: Homo sapiens
Genetic Insert: ATP10B
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11402; Vector Backbone:lentiviral transferplasmid; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32172343
Proper citation: RRID:Addgene_171770 Copy
Species: Homo sapiens
Genetic Insert: TUBG1
Vector Backbone Description: Backbone Marker:AMRAD corparation limited; Backbone Size:4900; Vector Backbone:pGEX2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34158617
Comments: The TUBG1 gene from the plasmid obtained from Jiri Bartek was subcloned into pGEX2t. Addgene NGS found mutation R275H [NP_001061.2 ] but the depositor confirmed that this mutation does not affect function
Proper citation: RRID:Addgene_171967 Copy
Species: Caenorhabditis elegans
Genetic Insert: his-58, eGFP, mCherry
Vector Backbone Description: Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33579952
Proper citation: RRID:Addgene_171845 Copy
Species: Homo sapiens
Genetic Insert: TUBG2
Vector Backbone Description: Backbone Marker:thermofisher; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34158617
Comments: The internal EcoRI was removed from the GeneCopeia mCherry tagged TUBG2/pReceiver-M56 plasmid and TUBG2 was sub-cloned into pcDNA3.1.
Proper citation: RRID:Addgene_171966 Copy
Species: Homo sapiens
Genetic Insert: F-box and leucine rich repeat protein 7
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32839549
Proper citation: RRID:Addgene_171848 Copy
Species: Homo sapiens
Genetic Insert: INPP5K
Vector Backbone Description: Vector Backbone:pCS Cherry DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171950 Copy
Species: Mus musculus
Genetic Insert: Mouse PE sequence (mPE)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-Promoter; Vector Types:Mammalian Expression, Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33458694
Proper citation: RRID:Addgene_172005 Copy
Species: Xenopus laevis
Genetic Insert: XDpr
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pGAD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: Description: Dpr yeast clone in original prey vector. 1.6kb EcoR1 fragment corresponds to C-terminal 1.2 kb of Xenopus Dpr and 3' UTR in pGAD vector.
Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_17191 Copy
Species: Xenopus laevis
Genetic Insert: XDpr
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: Description: Coding sequence of original XDpr prey clone (M35) cloned into pGEX vector as a 1.2 kb EcoR1-Xba1 fragment (Xba site is DAM methylated).
Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_17192 Copy
Species: Homo sapiens
Genetic Insert: Chibby
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6600; Vector Backbone:pMAL-c2E; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The full coding sequence of human Chibby, including 3' UTR was amplified by PCR, digested with BglII and Xho1, and subcloned into the BamH1 and Sal1 sites of pMAL-c2E. The TEV protease site was included in the 5'primer. Insert size ~1kb.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_17195 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:0; Vector Backbone:pCMV pGL3 Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: This is a home-cooked PGL3 Luciferase expression vector.
Reference: Ajamete Kaykas.
Proper citation: RRID:Addgene_17186 Copy
Vector Backbone Description: Backbone Size:5343; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 2005. Fire Lab Miniprep Number pPD176.36, Ligation number L6637.
Proper citation: RRID:Addgene_1720 Copy
Species: Homo sapiens
Genetic Insert: Human PE sequence (hPE)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-Promoter; Vector Types:Mammalian Expression, Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33458694
Proper citation: RRID:Addgene_172003 Copy
Species: Homo sapiens
Genetic Insert: FKBP DD (L106P)-UbK0G75C
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33007216
Proper citation: RRID:Addgene_172001 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc17 and RBSc21
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171909 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc45 and RBSc249
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171906 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc25 and RBSc249
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171905 Copy
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