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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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p663-UBC-miniTurbo-V5-eGFP_IDG-K Resource Report Resource Website |
RRID:Addgene_135263 | miniTurbo-V5-eGFP | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:52 | 0 | ||
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pHAGE-CMV-Flag-hcRed_IDG-K Resource Report Resource Website |
RRID:Addgene_135274 | Flag-hcRed | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Size:9763; Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:52 | 0 | ||
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pLBS.CAG Resource Report Resource Website |
RRID:Addgene_129349 | unspecified | Other | Ampicillin | Depositor Adm #1132 | Vector Backbone:pLBS.CAG; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:56 | 0 | ||
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pAAV-CAG-DIO-(STChRger2-TS-EYFP) Resource Report Resource Website |
RRID:Addgene_129394 | soma targeted ChRger2 | Other | Ampicillin | PMID:31611694 | Depositor has evidence that soma-targeted constructs may have different optical properties from their parent ChRgers and they have not been fully characterized. Users should be aware that they are beta-testing. | Backbone Size:5457; Vector Backbone:pAAV CAG DIO WPRE pA; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:57 | 0 | |
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pAAV-hSyn-STChRger2-TS-EYFP Resource Report Resource Website |
RRID:Addgene_129395 | soma targeted ChRger2 | Other | Ampicillin | PMID:31611694 | Depositor has evidence that soma-targeted constructs may have different optical properties from their parent ChRgers and they have not been fully characterized. Users should be aware that they are beta-testing. | Backbone Size:4559; Vector Backbone:pAAV hSyn WPRE pA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:57 | 0 | |
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p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS Resource Report Resource Website |
RRID:Addgene_129532 | codon-optimized AcrIIA4 | Other | Ampicillin | PMID:31439808 | Vector Backbone:scAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:59 | 0 | ||
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p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS Resource Report Resource Website 1+ mentions |
RRID:Addgene_129531 | codon-optimized AcrIIC3Nme | Other | Ampicillin | PMID:31439808 | Vector Backbone:scAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:58 | 1 | ||
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3TERM_35S Resource Report Resource Website |
RRID:Addgene_136114 | 3TERM_35S (CaMV) | Other | Chloramphenicol | PMID:32163700 | Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint. | Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | BsaI/ SapI domesticated | 2026-08-15 01:06:02 | 0 |
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3TERM_Nos Resource Report Resource Website |
RRID:Addgene_136115 | 3TERM_Nos (A. tumefaciens) | Other | Chloramphenicol | PMID:32163700 | Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint. | Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | BsaI/ SapI domesticated | 2026-08-15 01:06:02 | 0 |
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HP138-puro Resource Report Resource Website 1+ mentions |
RRID:Addgene_134246 | Contains Cas9 | Other | Ampicillin | PMID:28216383 | Vector Backbone:unknown; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:43 | 2 | ||
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S3_001_026_096 Resource Report Resource Website |
RRID:Addgene_134402 | mCherry | Other | Kanamycin | PMID:31433622 | Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | none | 2026-08-15 01:05:44 | 0 | |
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YFP-ICP0-C3 Resource Report Resource Website |
RRID:Addgene_134559 | ICP0 | Other | Kanamycin | PMID:22976303 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:05:45 | 0 | ||
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YFP-ICP0FxE-C3 Resource Report Resource Website |
RRID:Addgene_134561 | ICP0 | Other | Kanamycin | PMID:22976303 | YFP-ICP0FxE-C3 contains a V886A difference compared to the reference sequence. The functional outcome is unknown but this enzyme is catalytically inert as designed. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | RING finger deletion (FXE) | 2026-08-15 01:05:45 | 0 |
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pHCKan-yibDp-cimA3.7 Resource Report Resource Website |
RRID:Addgene_134595 | cimA3.7 | Other | Kanamycin | PMID:32441815 | Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint. | Vector Backbone:pTWIST-Kan-High Copy; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | I47V, E114V, H126Q, T204A, L238S | 2026-08-15 01:05:46 | 0 |
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pHCKan-yibDp-SBS-mCherry Resource Report Resource Website |
RRID:Addgene_134598 | SBS-mCherry | Other | Chloramphenicol | PMID:32441815 | Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint. | Backbone Marker:Twist Biosciences; Vector Backbone:pTWIST-Chlor-MC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:05:46 | 0 | |
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pVDM1001 Resource Report Resource Website |
RRID:Addgene_134660 | CRISPR guide RNA array | Other | Spectinomycin | PMID:31905238 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:Willem van Schaik; Backbone Size:4172; Vector Backbone:pWS3; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:05:47 | 0 | |
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BII-ChPtW-smURFP Resource Report Resource Website |
RRID:Addgene_133399 | smURFP-NLS | Other | Ampicillin | Backbone Marker:Madison Lab; Vector Backbone:BII-ChPtW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | N/A | 2026-08-15 01:05:35 | 0 | ||
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BII-STAR-tdT Resource Report Resource Website |
RRID:Addgene_133396 | tdTomato-NLS | Other | Ampicillin | Backbone Marker:Madison Lab; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | N/A | 2026-08-15 01:05:35 | 0 | ||
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BII-TOP-tdT Resource Report Resource Website |
RRID:Addgene_133395 | tdTomato-NLS | Other | Ampicillin | PMID:30017821 | Backbone Marker:Madison Lab; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | N/A | 2026-08-15 01:05:35 | 0 | |
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pAAV2-GCaMP6f-P2A-PAmCherry Resource Report Resource Website |
RRID:Addgene_133412 | GCaMP6f-P2A-PAmCherry | Other | Ampicillin | PMID:31831669 | pAAV2 : The Hope Center Viral Vectors Core at Washington University in St. Louis GCaMP6f :pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755 ; http://n2t.net/addgene:40755 ; RRID:Addgene_40755) PAmCherry: pPAmCherry1-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 31929 ; http://n2t.net/addgene:31929 ; RRID:Addgene_31929) | Backbone Size:4632; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:35 | 0 |
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