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Species: Other
Genetic Insert: miniTurbo-V5-eGFP
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135263 Copy
Species: Other
Genetic Insert: Flag-hcRed
Vector Backbone Description: Backbone Size:9763; Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135274 Copy
Species: Other
Genetic Insert: unspecified
Vector Backbone Description: Vector Backbone:pLBS.CAG; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Depositor Adm #1132
Proper citation: RRID:Addgene_129349 Copy
Species: Other
Genetic Insert: soma targeted ChRger2
Vector Backbone Description: Backbone Size:5457; Vector Backbone:pAAV CAG DIO WPRE pA; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31611694
Comments: Depositor has evidence that soma-targeted constructs may have different optical properties from their parent ChRgers and they have not been fully characterized. Users should be aware that they are beta-testing.
Proper citation: RRID:Addgene_129394 Copy
Species: Other
Genetic Insert: soma targeted ChRger2
Vector Backbone Description: Backbone Size:4559; Vector Backbone:pAAV hSyn WPRE pA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31611694
Comments: Depositor has evidence that soma-targeted constructs may have different optical properties from their parent ChRgers and they have not been fully characterized. Users should be aware that they are beta-testing.
Proper citation: RRID:Addgene_129395 Copy
Species: Other
Genetic Insert: codon-optimized AcrIIA4
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31439808
Proper citation: RRID:Addgene_129532 Copy
Species: Other
Genetic Insert: codon-optimized AcrIIC3Nme
Vector Backbone Description: Vector Backbone:scAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31439808
Proper citation: RRID:Addgene_129531 Copy
Species: Other
Genetic Insert: 3TERM_35S (CaMV)
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136114 Copy
Species: Other
Genetic Insert: 3TERM_Nos (A. tumefaciens)
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136115 Copy
Species: Other
Genetic Insert: Contains Cas9
Vector Backbone Description: Vector Backbone:unknown; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28216383
Proper citation: RRID:Addgene_134246 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Proper citation: RRID:Addgene_134402 Copy
Species: Other
Genetic Insert: ICP0
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22976303
Proper citation: RRID:Addgene_134559 Copy
Species: Other
Genetic Insert: ICP0
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22976303
Comments: YFP-ICP0FxE-C3 contains a V886A difference compared to the reference sequence. The functional outcome is unknown but this enzyme is catalytically inert as designed.
Proper citation: RRID:Addgene_134561 Copy
Species: Other
Genetic Insert: cimA3.7
Vector Backbone Description: Vector Backbone:pTWIST-Kan-High Copy; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32441815
Comments: Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_134595 Copy
Species: Other
Genetic Insert: SBS-mCherry
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTWIST-Chlor-MC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32441815
Comments: Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_134598 Copy
Species: Other
Genetic Insert: CRISPR guide RNA array
Vector Backbone Description: Backbone Marker:Willem van Schaik; Backbone Size:4172; Vector Backbone:pWS3; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31905238
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_134660 Copy
Species: Other
Genetic Insert: smURFP-NLS
Vector Backbone Description: Backbone Marker:Madison Lab; Vector Backbone:BII-ChPtW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_133399 Copy
Species: Other
Genetic Insert: tdTomato-NLS
Vector Backbone Description: Backbone Marker:Madison Lab; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_133396 Copy
Species: Other
Genetic Insert: tdTomato-NLS
Vector Backbone Description: Backbone Marker:Madison Lab; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30017821
Proper citation: RRID:Addgene_133395 Copy
Species: Other
Genetic Insert: GCaMP6f-P2A-PAmCherry
Vector Backbone Description: Backbone Size:4632; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31831669
Comments: pAAV2 : The Hope Center Viral Vectors Core at Washington University in St. Louis
GCaMP6f :pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755 ; http://n2t.net/addgene:40755 ; RRID:Addgene_40755)
PAmCherry: pPAmCherry1-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 31929 ; http://n2t.net/addgene:31929 ; RRID:Addgene_31929)
Proper citation: RRID:Addgene_133412 Copy
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