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Species: Synthetic
Genetic Insert: FKBP Degron Domain + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: FKBP degron domain is stabilized by the ligand Shld1
Proper citation: RRID:Addgene_121789 Copy
Species: Synthetic
Genetic Insert: Sa-dCas9/KRAB (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: Sa-dCas9 PCR'd from Addgene #68495, KRAB PCR'd from Addgene #60954
Proper citation: RRID:Addgene_121823 Copy
Species: Synthetic
Genetic Insert: Sa-Cas9 (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: PCR'd from Addgene #61591
Proper citation: RRID:Addgene_121827 Copy
Species: Synthetic
Genetic Insert: x-dCas9(3.7) (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: x-dCas9(3.7) PCR'd from Addgene #108383
Proper citation: RRID:Addgene_121828 Copy
Species: Synthetic
Genetic Insert: P2A-Neo + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121781 Copy
Species: Synthetic
Genetic Insert: P2A-Hygro + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121782 Copy
Species: Synthetic
Genetic Insert: P2A-Zeo + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121783 Copy
Species: Synthetic
Genetic Insert: P2A-Blast + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121784 Copy
Species: Synthetic
Genetic Insert: P2A-Puro + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121785 Copy
Species: Synthetic
Genetic Insert: P2A-Hygro + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121787 Copy
Species: Synthetic
Genetic Insert: hU6::SaCas9 gRNA scaffold + RIP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. SaCas9 gRNA scaffold derived from PMID: 26721686
Proper citation: RRID:Addgene_121820 Copy
Species: Synthetic
Genetic Insert: P2A-eYFP + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121778 Copy
Species: Synthetic
Genetic Insert: mU6::SaCas9 gRNA scaffold
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. SaCas9 gRNA scaffold derived from PMID: 26721686
Proper citation: RRID:Addgene_121811 Copy
Species: Synthetic
Genetic Insert: P2A-Blast + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121779 Copy
Species: Synthetic
Genetic Insert: hU6::SaCas9 gRNA scaffold + CMV promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. SaCas9 gRNA scaffold derived from PMID: 26721686
Proper citation: RRID:Addgene_121813 Copy
Species: Synthetic
Genetic Insert: hU6::SaCas9 gRNA scaffold + EF1a promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. SaCas9 gRNA scaffold derived from PMID: 26721686
Proper citation: RRID:Addgene_121814 Copy
Species: Synthetic
Genetic Insert: hU6::SaCas9 gRNA scaffold + RIP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. SaCas9 gRNA scaffold derived from PMID: 26721686
Proper citation: RRID:Addgene_121815 Copy
Species: Synthetic
Genetic Insert: mU6::xCas9(3.7) gRNA scaffold
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. x/SpCas9 gRNA scaffold derived from PMID: 26671237
Proper citation: RRID:Addgene_121816 Copy
Species: Synthetic
Genetic Insert: P2A-eYFP + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121770 Copy
Species: Synthetic
Genetic Insert: eGFP + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30590691
Proper citation: RRID:Addgene_121771 Copy
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