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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAAV-CMV-mTurquoise2-NLS Resource Report Resource Website 1+ mentions |
RRID:Addgene_165443 | mTurquoise2-NLS | Other | Ampicillin | PMID:34176926 | Backbone Marker:Esteban Engel lab; Vector Backbone:pAAV-CMV-EGFP-NLS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 1 | ||
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pOpen-GBDpol Resource Report Resource Website |
RRID:Addgene_165501 | Pyrococcus Sp. Heat-Stable DNA Polymerase | Other | Ampicillin | Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Comparable to Deep Vent at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pOpen-9N7polA Resource Report Resource Website |
RRID:Addgene_165500 | 9 degrees N-7 DNA polymerase | Other | Ampicillin | Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity (1-5% of the wildtype). Features a half-life of 6.7 hours at 95 degrees C. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pOpen-T7gene1 Resource Report Resource Website |
RRID:Addgene_165505 | T7 RNA Polymerase | Other | Ampicillin | Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Plays also a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pOpen-Eco31I Resource Report Resource Website |
RRID:Addgene_165506 | Eco31I | Other | Ampicillin | Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pOpen-EcoRIR Resource Report Resource Website |
RRID:Addgene_165504 | EcoRI | Other | Ampicillin | Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pOpen-TEV protease Resource Report Resource Website |
RRID:Addgene_165509 | TEV protease | Other | Ampicillin | Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pOpen-NotIR (GC) Resource Report Resource Website |
RRID:Addgene_165507 | NotI (GC-rich recognition site) | Other | Ampicillin | Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pOpen-DNA Topoisomerase 1B Vaccinia Virus Resource Report Resource Website |
RRID:Addgene_165510 | DNA Topoisomerase 1B Vaccinia Virus | Other | Ampicillin | Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the DNA and the topoisomerase. If a 5' hydroxyl group from a different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands and releasing topoisomerase. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pOpen-BsupolLF Resource Report Resource Website |
RRID:Addgene_165563 | Bsu DNA Polymerase I, Large Fragment | Other | Ampicillin | Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-ECOligA Resource Report Resource Website |
RRID:Addgene_165564 | E. coli DNA Ligase | Other | Ampicillin | Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-Endonuclease VIII E. coli Resource Report Resource Website |
RRID:Addgene_165561 | Endonuclease VIII E. coli | Other | Ampicillin | Used in the preCR mix from NEB; Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-BstpolLF Resource Report Resource Website |
RRID:Addgene_165553 | Bst DNA Polymerase, Large Fragment | Other | Ampicillin | Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement. Additionally used for Loop-Mediated Isothermal Amplification (LAMP), DNA sequencing through high GC regions, rapid sequencing from nanogram amounts of DNA template. Can be used in the standard Sanger one step or two step protocol which separates the labelling reaction from the elongation termination reaction. The enzyme can be used in double stranded sequencing. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-SapIM2 Resource Report Resource Website |
RRID:Addgene_165557 | M2.SapI | Other | Ampicillin | Site-specific DNA methyltransferase 2; protects DNA from cleavage by SapI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-T4gene30 Resource Report Resource Website |
RRID:Addgene_165554 | T4-DNA Ligase | Other | Ampicillin | Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-ALPI Resource Report Resource Website |
RRID:Addgene_165555 | CIP (calf intestinal phosphatase) | Other | Ampicillin | CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-T5gene12/13 Resource Report Resource Website |
RRID:Addgene_165559 | T5 Exonuclease | Other | Ampicillin | Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction. Ideal for removal of incomplete ligation products from ligated circular double-stranded DNA, degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning, and degradation of contaminating linear and nicked DNA in plasmid samples. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-BovDNTT Resource Report Resource Website |
RRID:Addgene_165580 | Bovine DNTT | Other | Ampicillin | Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-HSlig3 Resource Report Resource Website |
RRID:Addgene_165574 | DNA Ligase III | Other | Ampicillin | Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992)). The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-phi29pol Resource Report Resource Website |
RRID:Addgene_165573 | phi29 DNA Polymerase | Other | Ampicillin | DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 |
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