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Species: Synthetic
Genetic Insert: 90.a
Vector Backbone Description: Vector Backbone:pSTC0-zeo; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38902262
Comments: Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
Proper citation: RRID:Addgene_202336 Copy
Species: Synthetic
Genetic Insert: 98.a
Vector Backbone Description: Vector Backbone:pSTC0-zeo; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38902262
Comments: Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
Proper citation: RRID:Addgene_202332 Copy
Species: Synthetic
Genetic Insert: opt.b
Vector Backbone Description: Vector Backbone:pSTC0-zeo; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38902262
Comments: Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
Note: Addgene NGS results identified a SNP in the plasmid relative to the depositor's reference sequence. The depositor has confirmed that the discrepancy is not expected to have an impact on plasmid function.
Proper citation: RRID:Addgene_202348 Copy
Species: Synthetic
Genetic Insert: rw-consensus
Vector Backbone Description: Vector Backbone:pSTC0-zeo; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38902262
Comments: Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
Proper citation: RRID:Addgene_202349 Copy
Species: Synthetic
Genetic Insert: 50.a
Vector Backbone Description: Vector Backbone:pSTC0-zeo; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38902262
Comments: Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
Proper citation: RRID:Addgene_202342 Copy
Species: Synthetic
Genetic Insert: 70.b
Vector Backbone Description: Vector Backbone:pSTC0-zeo; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38902262
Comments: Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
Proper citation: RRID:Addgene_202341 Copy
Species: Synthetic
Genetic Insert: 50.b
Vector Backbone Description: Vector Backbone:pSTC0-zeo; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38902262
Comments: Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
Proper citation: RRID:Addgene_202343 Copy
Species: Synthetic
Genetic Insert: TEM-1
Vector Backbone Description: Vector Backbone:pSTC0-zeo; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38902262
Comments: Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
Proper citation: RRID:Addgene_202351 Copy
Vector Backbone Description: Backbone Size:1986; Vector Backbone:pREMI-Z; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:11856375
Comments: Linearize at BamHI.
Proper citation: RRID:Addgene_59527 Copy
Species: Homo sapiens
Genetic Insert: Anti-Ebola 13C6 monoclonal antibody
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:28786662
Proper citation: RRID:Addgene_85935 Copy
Species: Homo sapiens
Genetic Insert: Anti-Ebola 4G7 monoclonal antibody
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:28786662
Proper citation: RRID:Addgene_85934 Copy
Species: Mus musculus
Genetic Insert: MYO10
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2900; Vector Backbone:pIZ/V5-His; Vector Types:Insect Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27932498
Proper citation: RRID:Addgene_87257 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRIC-B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3222; Vector Backbone:pPICZ C; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27698420
Comments: The vector pPICZ C and insert sequence were modified as follows:
1. Restriction sites including Pml I; Sfi I; BsmB I; Asp718 I; Kpn I were destroyed during cloning full-length CeTRIC-B2 to the vector.
2. In the C-terminal truncation, the sequence between XhoI and polyhistidine had been deleted. That is to say, restriction sites including Xho I, Sac II, Not I, Sna B I, and myc-epitope, were destroyed during truncation.
3. A kozak suqence "ACC" was inserted between the restriction site "EcoR I" and initial codon, we added these 3 bp into insert sequence size.
In summary, the size of pPICZ C backbone is changed from 3329 bp to be 3222 bp.
Proper citation: RRID:Addgene_87345 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78937 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78939 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78935 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78941 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78942 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78974 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78973 Copy
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