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Species: Saccharomyces cerevisiae
Genetic Insert: GAL4::VP16
Vector Backbone Description: Backbone Size:8770; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26228 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9223; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: nlsLexA::GADfl
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26232 Copy
Species: Homo sapiens
Genetic Insert: p65AD-Zip
Vector Backbone Description: Backbone Size:8731; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26234 Copy
Species: Homo sapiens
Genetic Insert: nlsLexA::p65
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26230 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:9116; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced into a Tet-On Advanced cell line for expression of the GOI.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.
Proper citation: RRID:Addgene_26434 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:9402; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI.
Proper citation: RRID:Addgene_26430 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21344536
Proper citation: RRID:Addgene_26360 Copy
Genetic Insert: attR4-ccdB-attR2
Vector Backbone Description: Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:15489340
Comments: A KpnI-ApaI fragment containing the GFP encoding sequence and the unc-54 3′ UTR from pPD95.79 was cloned into pDP#MM016b. An EcoRV fragment of the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into this new construct digested with Acc65I and filled in with Klenow.
The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.
Proper citation: RRID:Addgene_26415 Copy
Genetic Insert: attR4-ccdB-attR2 cassette
Vector Backbone Description: Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:15489340
Comments: A HindIII-KpnI fragment containing the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into the unc-119 rescue vector pDP#MM016b.
The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.
Proper citation: RRID:Addgene_26414 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9637; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20205919
Comments: Two mutations are present in the LEU2 sequence: D52E and V69A
Proper citation: RRID:Addgene_20161 Copy
Species: cloning vector
Genetic Insert: pWS-TK3
Vector Backbone Description: Backbone Size:6379; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18546598
Comments: Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.
Proper citation: RRID:Addgene_20349 Copy
Species: cloning vector
Genetic Insert: pWS-TK6
Vector Backbone Description: Backbone Size:6355; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18546598
Comments: Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.
Proper citation: RRID:Addgene_20350 Copy
Species: bacteria
Genetic Insert: aphA::cat
Vector Backbone Description: Backbone Marker:Skorupski, K., Taylor, R.K.; Backbone Size:4400; Vector Backbone:pKAS32; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16750581
Comments: Contains cat which confers resistance to chloramphenicol.
Proper citation: RRID:Addgene_20870 Copy
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:6465; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19252478
Proper citation: RRID:Addgene_20960 Copy
Genetic Insert: GateWay conversion cassette
Vector Backbone Description: Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19014667
Comments: pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4
for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation.
Stbl3 cells are advisable to use after users have done the LR recombination reaction.
See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html
for more information and protocols for this plasmid.
Further advice from the depositing lab:
"Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive...
- Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay"
Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct.
Proper citation: RRID:Addgene_21189 Copy
Vector Backbone Description: Backbone Size:3744; Vector Backbone:pBPXcm-1; Vector Types:Plant expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_33298 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms.
This vector does not contain a visual marker on the backbone and can be used when the gene of interest has visual phenotype.
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34914 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Puromycin resistance vector for drug selection in worms.
This vector replaces pBCN22-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34918 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Puromycin resistance vector for drug selection in worms.
This vector replaces pBCN21-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34917 Copy
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