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Vector Backbone Description: Vector Backbone:pGGP-AG; Vector Types:Chromoprotein-marked pVS1 backbone; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:41415724
Comments: Please visit https://doi.org/10.1101/2024.08.04.606528 for bioRxiv preprint.
Proper citation: RRID:Addgene_222076 Copy
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:unknown; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54404 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-S (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: ihpRNA silencing vector (binary expression vector)
Proper citation: RRID:Addgene_54403 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54364 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-S (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54347 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54357 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-S (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54356 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:6005; Vector Backbone:pGreen III; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51777 Copy
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:19366901
Proper citation: RRID:Addgene_48257 Copy
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:19366901
Proper citation: RRID:Addgene_48255 Copy
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:19366901
Proper citation: RRID:Addgene_48256 Copy
Vector Backbone Description: Backbone Size:2673; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24376629
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 602-612bp
BsaI site #2 = 2040-2050bp
Proper citation: RRID:Addgene_48869 Copy
Vector Backbone Description: Backbone Size:2673; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24376629
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 640-650bp
BsaI site #2 = 2078-2088bp
Proper citation: RRID:Addgene_48868 Copy
Species: Synthetic
Genetic Insert: RSF1010 ORI plus NGS barcode 14, spectinomycin resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251940 Copy
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