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Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B2;C0)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34656 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B2;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34659 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B1;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34658 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3 GFP H2B tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Comments: We would like to thank Andrew Wood and Barbara Meyer for sending us the Peft-3 promoter and pointing out how strong the promoter is.
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_34877 Copy
Species: Caenorhabditis elegans
Genetic Insert: tbb-2 gld-1 operon
Vector Backbone Description: Vector Backbone:pDONRP2-P3R; Vector Types:Worm targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34875 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mos1 transpoase
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_34874 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34866 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34865 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34863 Copy
Species: Caenorhabditis elegans
Genetic Insert: gamma-tubulin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Comments: 1st generation pie-1 vector
Proper citation: RRID:Addgene_26948 Copy
Species: Caenorhabditis elegans
Genetic Insert: lsm-3
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26944 Copy
Species: Caenorhabditis elegans
Genetic Insert: histone H2B
Vector Backbone Description: Backbone Size:0; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Comments: 1st generation pie-1 vector
Note that there are some discrepancies between the Addgene QC sequence and the depositor's sequence. They should not affect function.
Proper citation: RRID:Addgene_26946 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmyo-3::dsRed::let-858_3'UTR
Vector Backbone Description: Backbone Size:2500; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Proper citation: RRID:Addgene_27511 Copy
Species: Caenorhabditis elegans
Genetic Insert: pREAR::lin-4::unc-54_3'UTR
Vector Backbone Description: Backbone Size:4078; Vector Backbone:n/a; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Comments: Please note that there are some slight discrepancies between Addgene's quality control sequence and the theoretical sequence from the Fire lab. These differences should not affect function.
Proper citation: RRID:Addgene_27508 Copy
Species: Caenorhabditis elegans
Genetic Insert: pREAR::DsRed::unc-54_3'UTR
Vector Backbone Description: Backbone Size:2500; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Proper citation: RRID:Addgene_27509 Copy
Species: Caenorhabditis elegans
Genetic Insert: pcol-10::lin-4::let-858_3'UTR
Vector Backbone Description: Backbone Size:6167; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Comments: There are some discrepancies between Addgene's quality controls sequence and the depositor's sequence, but the Fire lab does not believe they will affect the function of the plasmid.
Proper citation: RRID:Addgene_27506 Copy
Species: Caenorhabditis elegans
Genetic Insert: phlh-8::lin-4::unc-54_3'UTR
Vector Backbone Description: Backbone Size:4972; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Comments: There are some discrepancies between Addgene's quality controls sequence and the depositor's sequence, but the Fire lab does not believe they will affect the function of the plasmid.
Proper citation: RRID:Addgene_27507 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmyo-3::yfp::lin-14_3'UTR
Vector Backbone Description: Backbone Size:2450; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Proper citation: RRID:Addgene_27505 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_28091 Copy
Species: Caenorhabditis elegans
Genetic Insert: BiP
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: This reporter gene tracks the expression of endogenous BiP (hsp-4) in C. elegans. It consists of a ~1.1 KB genomic C. elegans fragment linked to GFP in the vector pPD95.75. The fusion between the GFP artificial gene and hsp-4 takes place at AA #3 of HSP-4)
Proper citation: RRID:Addgene_21896 Copy
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