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Species: Gallus gallus
Genetic Insert: Fz 7
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(B); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862751
Comments: Frizzled 7 wild type ORF: 5’ BsaI-primer, 3’ EcoRI-primer,
shuttle vector: Slax13.
Proper citation: RRID:Addgene_13930 Copy
Species: Gallus gallus
Genetic Insert: Fz 7
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862751
Comments: Frizzled 7 wild type ORF: 5’ BsaI-primer, 3’ EcoRI-primer,
shuttle vector: Slax13.
Proper citation: RRID:Addgene_13929 Copy
Species: Gallus gallus
Genetic Insert: pitx2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9708733
Comments: full length original library isolate from Mackem dT, stage18-24 limb bud library. Used as an anti-sense riboprobe template and for subcloning.
Proper citation: RRID:Addgene_13927 Copy
Species: Gallus gallus
Genetic Insert: pitx2
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9708733
Comments: The entire open reading frame of cPitx2 was generated by high fidelity PCR and cloned into a version of pSlax13 containing a triple HA (Flu) tag (Laufer et al., 1997). The sequence of the PCR-generated insert was confirmed by sequencing. A ClaI fragment containing the cPitx2-Flu sequence was cloned into the RCAS retroviral vector.
Proper citation: RRID:Addgene_13928 Copy
Species: Gallus gallus
Genetic Insert: Fz 7
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862751
Comments: Frizzled 7 C-terminal 24 AA truncated: 5’ BsaI-primer, 3’ EcoRI-primer,
shuttle vector: Slax13.
Proper citation: RRID:Addgene_13933 Copy
Species: Gallus gallus
Genetic Insert: Gdf5
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11239392
Comments: Partial chick Gdf 5 gene (RT-PCR product using degenerate primers, ~400bp fragment corresponding to AA 285-411 of mouse GDF5).
Addgene QC NGS analysis identifies an E193G discrepancy relative to reference sequence ASW22707.1.
Proper citation: RRID:Addgene_13944 Copy
Species: Gallus gallus
Genetic Insert: Aggrecan
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11239392
Comments: 687 bp partial clone of chick aggrecan, generated by RT-PCR corresponding to nucleotides 113-800 of Genbank sequence #L21913.
Proper citation: RRID:Addgene_13945 Copy
Species: Gallus gallus
Genetic Insert: CD44 related
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11239392
Comments: 470 bp partial cDNA clone of chick CD44rel amplified by RT-PCR corresponding to nucleotides 814-1284 of Genbank sequence #AF153205.
NOTE: Has a R323Q mutation compared GenBank. This mutation was not intentional, but it is not known to effect function.
Proper citation: RRID:Addgene_13946 Copy
Species: Gallus gallus
Genetic Insert: Gli3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11044624
Comments: For in situ probe; 3’ EcoRI fragment of cGli3 including the stop, does not contain the zinc finger region (denoted 19a1.5).
Proper citation: RRID:Addgene_13953 Copy
Species: Gallus gallus
Genetic Insert: Lrdr
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12097899
Proper citation: RRID:Addgene_13950 Copy
Species: Gallus gallus
Genetic Insert: Nkx2.5
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10934012
Comments: Entire coding region of Nkx5.2.
Proper citation: RRID:Addgene_13951 Copy
Species: Gallus gallus
Genetic Insert: SugarCrisp
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11287197
Comments: SugarCrisp cDNA ~1kb Cla I fragment derived from pMIW/Sugar Crisp.
Proper citation: RRID:Addgene_13956 Copy
Species: Gallus gallus
Genetic Insert: Scleraxis
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11585810
Comments: cScx digested by EcoRV, Bgl II and religated; ~580bp (80 bp of coding plus 500 bp of UTR).
Proper citation: RRID:Addgene_13957 Copy
Species: Gallus gallus
Genetic Insert: LefX
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12871700
Proper citation: RRID:Addgene_13972 Copy
Species: Gallus gallus
Genetic Insert: Tbx5
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12736217
Comments: Contains amino acids 1-274 of the full-length Tbx5 clone fused to amino acids 2-298 of the Drosophila Engrailed protein.
Proper citation: RRID:Addgene_13967 Copy
Species: Gallus gallus
Genetic Insert: Tbx5
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12736217
Comments: Tbx5 D1 truncated (c-terminal truncation), putative dominant negative.
Proper citation: RRID:Addgene_13968 Copy
Species: Gallus gallus
Genetic Insert: Lunatic Fringe
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121552
Proper citation: RRID:Addgene_13977 Copy
Species: Gallus gallus
Genetic Insert: Wnt10a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12871700
Comments: 400 bp fragment of cWnt10a generated by degenerate PCR, for in situ.
Proper citation: RRID:Addgene_13973 Copy
Species: Gallus gallus
Genetic Insert: Lunatic Fringe
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121552
Comments: Flag epitope is a Nhe I - BamHI fragment attached to 3' end of lunatic fringe.
Proper citation: RRID:Addgene_13979 Copy
Species: Gallus gallus
Genetic Insert: Radical Fringe
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(B); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121552
Comments: Flag epitope is a Nhe I - BamHI fragment attached to 3' end of radical fringe.
Proper citation: RRID:Addgene_13983 Copy
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