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Species: Mus musculus
Genetic Insert: PGC-1alpha Isoform1
Vector Backbone Description: Backbone Size:5424; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23217713
Comments: Note that Addgene NGS results identified a Q339K mutation relative to the canonical GenBank sequence. This mutation has not been reported to affect function.
Proper citation: RRID:Addgene_45501 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP720
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4067; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45460 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45462 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP702
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4074; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23770755
Proper citation: RRID:Addgene_45455 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4012; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23770755
Comments: For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/
Proper citation: RRID:Addgene_45457 Copy
Species: Homo sapiens
Genetic Insert: ATRX
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24834375
Comments: The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid.
Proper citation: RRID:Addgene_45444 Copy
Species: Rattus norvegicus
Genetic Insert: NR1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45446 Copy
Species: Rattus norvegicus
Genetic Insert: NR2A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45445 Copy
Species: Synthetic
Genetic Insert: AcrB/AcrD/AcrFa, ABO_0964
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45434 Copy
Species: Homo sapiens
Genetic Insert: hM3D (Gq)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5059; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360345
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_45547 Copy
Species: jellyfish
Genetic Insert: mit-2mutAEQ
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22671130
Comments: Note that numbering of the aequorin mutations in this plasmid is relative to the valine at position 8 in the original sequence from Inouye et al., PNAS 82, 3154 (1985) PubMed ID: 3858813. When compared to this sequence (GenBank ID AAA27720.1) the mutations at N28L and D119A would correspond to N35L and D126A.
Proper citation: RRID:Addgene_45539 Copy
Species: Mus musculus
Genetic Insert: alpha Klotho
Vector Backbone Description: Backbone Size:5710; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26881702
Proper citation: RRID:Addgene_45532 Copy
Species: Synthetic
Genetic Insert: CAR-GECO1
Vector Backbone Description: Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23452507
Proper citation: RRID:Addgene_45493 Copy
Species: Synthetic
Genetic Insert: R-GECO1.2
Vector Backbone Description: Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23452507
Proper citation: RRID:Addgene_45494 Copy
Species: Homo sapiens
Genetic Insert: eIF4GI transcript variant 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19114555
Comments: For diagnostic digest, an additional PvuI digestion is necessary to distinguish the insert from the vector because of their similar sizes.
The EIF4G1 translation contains M432V compared to NP_937884 (NM_198241.2); however the nucleotide change resulting in the amino acid change is more conserved between species, and it is located between functional domains of eIF4G1 (PABP-binding site and eIF4E-binding site), and does not affect its function as a translational factor
Proper citation: RRID:Addgene_45640 Copy
Species: Homo sapiens
Genetic Insert: HDAC4
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17873280
Proper citation: RRID:Addgene_45636 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15048125
Comments: Alternate plasmid names: pD40-His-c-Myc; pcDNA-DEST40 Myc wt
This His6-tagged c-Myc plasmid was created by PCR amplification of the coding sequence for wild-type murine c-Myc2 from the CMVMyc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-Myc.
For plasmid usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971).
Proper citation: RRID:Addgene_45597 Copy
Species: Anabaena variabilis
Genetic Insert: Ava3855
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5293; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20813918
Proper citation: RRID:Addgene_45743 Copy
Genetic Insert: co I-Sce I
Vector Backbone Description: Backbone Size:5071; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121685
Proper citation: RRID:Addgene_45574 Copy
Species: Homo sapiens
Genetic Insert: eGFP with I-Sce I TS
Vector Backbone Description: Backbone Size:4483; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121685
Proper citation: RRID:Addgene_45575 Copy
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