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Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:4636; Vector Backbone:pCMV-7.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23942116
Comments: This plasmid can be used to isolate specific genomic regions of interest using a catalytically inactive Cas9 fused with a tag(s).
Purify: locus-specific chromatin immunoprecipitation (enChIP)
This system is compatible with gRNA_cloning_vector (www.addgene.org/41824) from the Church lab.
Additional information and protocols can be found at:
http://www.med.hirosaki-u.ac.jp/~bgb/iChIP_protocols/index_e.html
Proper citation: RRID:Addgene_47948 Copy
Species: Homo sapiens
Genetic Insert: CIN85
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751601
Comments: The CIN85 insert in this plasmid contains a D114G mutation when compared to GenBank accession number NP_114098.1. This mutation was not intentional and was present in the original CIN85 clone.
Proper citation: RRID:Addgene_47935 Copy
Species: Homo sapiens
Genetic Insert: Miro2 T18N
Vector Backbone Description: Backbone Marker:Dr. Alan Hall, MRC-LMCB; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630562
Proper citation: RRID:Addgene_47897 Copy
Species: Homo sapiens
Genetic Insert: Miro1 E208K/E328K
Vector Backbone Description: Backbone Marker:Dr. Alan Hall, MRC-LMCB; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630562
Proper citation: RRID:Addgene_47894 Copy
Species: Homo sapiens
Genetic Insert: Miro2
Vector Backbone Description: Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12482879
Proper citation: RRID:Addgene_47891 Copy
Species: Synthetic
Genetic Insert: T1ER
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23838183
Comments: Ratiometric calcium sensor targeted to ER with KDEL sequence.
The CFP fragment of Addgene plasmid 36325 pcDNA-D1ER was replaced with the brighter fluorescent protein mTurquoise.
Proper citation: RRID:Addgene_47928 Copy
Species: Synthetic
Genetic Insert: nls-zcas9-nls
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23918387
Comments: Note from depositor: Although this plasmid produces smaller transcripts in addition to the expected one, the product mixture displays activity comparable to that from pT3TS-nCas9n.
For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/
Proper citation: RRID:Addgene_47929 Copy
Genetic Insert: H2B-tdiRFP
Vector Backbone Description: Vector Backbone:pCAG-ME-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096363
Proper citation: RRID:Addgene_47884 Copy
Species: Homo sapiens
Genetic Insert: Miro1
Vector Backbone Description: Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12482879
Proper citation: RRID:Addgene_47888 Copy
Vector Backbone Description: Vector Backbone:pCAG-T7-TALEN (Sangamo); Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096363
Proper citation: RRID:Addgene_47875 Copy
Species: N meningitidis
Genetic Insert: NmCas9
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pSimpleII; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23940360
Proper citation: RRID:Addgene_47872 Copy
Genetic Insert: TALE-mClover
Vector Backbone Description: Vector Backbone:pTALYM3; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096363
Proper citation: RRID:Addgene_47878 Copy
Species: Homo sapiens
Genetic Insert: hCas9 with C. elegans 5' and 3' UTRs
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:Bluescript; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23979577
Proper citation: RRID:Addgene_47911 Copy
Genetic Insert: TALE-mRuby2
Vector Backbone Description: Vector Backbone:pTALYM4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096363
Proper citation: RRID:Addgene_47879 Copy
Vector Backbone Description: Vector Backbone:pCAG-T7-TALEN (Sangamo); Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096363
Proper citation: RRID:Addgene_47876 Copy
Species: N meningitidis
Genetic Insert: NmCas9
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pSimpleII; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23940360
Proper citation: RRID:Addgene_47869 Copy
Species: Homo sapiens
Genetic Insert: human LIN28A
Vector Backbone Description: Backbone Marker:Dr.Toshio Kitamura of the University of Tokyo; Backbone Size:4800; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23812749
Comments: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Proper citation: RRID:Addgene_47902 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: Infrared fluorescent protein
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Vector Backbone:pAAV CaMKII hChR2-EYFP; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_47903 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: Infrared fluorescent protein
Vector Backbone Description: Backbone Marker:NIDA OTTC; Vector Backbone:pAAV c-fos eYFP; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Comments: Note that the "N" in the depositor's sequences is legitimately ambiguous in both the Addgene stock and the depositor's original stock. This repeat region may vary in different preps of the plasmid, but it should not affect the plasmid function.
Proper citation: RRID:Addgene_47906 Copy
Species: Homo sapiens
Genetic Insert: Miro2 E208K/E328K
Vector Backbone Description: Backbone Marker:Dr. Alan Hall, MRC-LMCB; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630562
Proper citation: RRID:Addgene_47900 Copy
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