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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pcDNA3.1-Flag-PGC-1alpha1
 
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1+ mentions
RRID:Addgene_45501 PGC-1alpha Isoform1 Mus musculus Ampicillin PMID:23217713 Note that Addgene NGS results identified a Q339K mutation relative to the canonical GenBank sequence. This mutation has not been reported to affect function. Backbone Size:5424; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Q339K relative to NM_008904 2026-08-15 01:15:24 1
pBAD/HisB-iRFP720
 
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1+ mentions
RRID:Addgene_45460 iRFP720 Rhodopseudomonas palustris Ampicillin PMID:23770755 Backbone Marker:Invitrogen; Backbone Size:4067; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193K, F198Y, D202F, V254N, Y258F, A283V, K288T, N290Y relative to RpBphP2 2026-08-15 01:15:24 1
pMito-iRFP670
 
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1+ mentions
RRID:Addgene_45462 iRFP670 Rhodopseudomonas palustris Kanamycin PMID:23770755 Backbone Marker:Clontech; Backbone Size:4038; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T2A, S45T, V116I, D124E, M170L, K180M, D202L, I203V, I219V, R233H, V251I, V254C, Y258F, A283I, F307L relative to RpBphP6 2026-08-15 01:15:24 2
pBAD/HisB-iRFP702
 
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1+ mentions
RRID:Addgene_45455 iRFP702 Rhodopseudomonas palustris Ampicillin PMID:23770755 Backbone Marker:Invitrogen; Backbone Size:4074; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T2A, S45T, D124E, M170L, D202L, I203V, I219V, R233H, V251I, Y258F, A283I, F307L relative to RpBphP6 2026-08-15 01:15:24 1
piRFP670-N1
 
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50+ mentions
RRID:Addgene_45457 iRFP670 Rhodopseudomonas palustris Kanamycin PMID:23770755 For more information on in vivo imaging, please see: https://www.addgene.org/fluorescent_proteins/in_vivo/ Backbone Marker:Clontech; Backbone Size:4012; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T2A, S45T, V116I, D124E, M170L, K180M, D202L, I203V, I219V, R233H, V251I, V254C, Y258F, A283I, F307L relative to RpBphP6 2026-08-15 01:15:24 57
IF-GFP-ATRX
 
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1+ mentions
RRID:Addgene_45444 ATRX Homo sapiens Kanamycin PMID:24834375 The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin isoform 2, missing codon E124 2026-08-15 01:15:24 7
pCI-EGFP-NR1 wt
 
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1+ mentions
RRID:Addgene_45446 NR1 Rattus norvegicus Ampicillin PMID:12160751 Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin EGFP inserted after the predicted signal peptide cleavage site (after amino acid residue 26) 2026-08-15 01:15:24 7
pCI-EGFP-NR2a wt
 
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1+ mentions
RRID:Addgene_45445 NR2A Rattus norvegicus Ampicillin PMID:12160751 Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin EGFP inserted after the predicted signal peptide cleavage site (after amino acid residue 21) 2026-08-15 01:15:24 5
pBbA5k-EPL95
 
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1+ mentions
RRID:Addgene_45434 AcrB/AcrD/AcrFa, ABO_0964 Synthetic Kanamycin PMID:21556065 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:15:24 1
pcDNA5/FRT-HA-hM3D(Gq)
 
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10+ mentions
RRID:Addgene_45547 hM3D (Gq) Homo sapiens Ampicillin PMID:17360345 These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. Backbone Marker:Invitrogen; Backbone Size:5059; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:24 16
pcDNA3.1+/mit-2mutAEQ
 
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1+ mentions
RRID:Addgene_45539 mit-2mutAEQ jellyfish Ampicillin PMID:22671130 Note that numbering of the aequorin mutations in this plasmid is relative to the valine at position 8 in the original sequence from Inouye et al., PNAS 82, 3154 (1985) PubMed ID: 3858813. When compared to this sequence (GenBank ID AAA27720.1) the mutations at N28L and D119A would correspond to N35L and D126A. Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Asp119Ala and Asn28Leu 2026-08-15 01:15:25 5
pCMV-Kl-EGFP
 
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1+ mentions
RRID:Addgene_45532 alpha Klotho Mus musculus Ampicillin and Kanamycin PMID:26881702 Backbone Size:5710; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:15:24 1
CMV-CAR-GECO1
 
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1+ mentions
RRID:Addgene_45493 CAR-GECO1 Synthetic Ampicillin PMID:23452507 Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P 2026-08-15 01:15:24 3
CMV-R-GECO1.2
 
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10+ mentions
RRID:Addgene_45494 R-GECO1.2 Synthetic Ampicillin PMID:23452507 Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M/L419I 2026-08-15 01:15:24 25
pcDNA3 HA eIF4GI (1-1599)
 
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1+ mentions
RRID:Addgene_45640 eIF4GI transcript variant 2 Homo sapiens Ampicillin PMID:19114555 For diagnostic digest, an additional PvuI digestion is necessary to distinguish the insert from the vector because of their similar sizes. The EIF4G1 translation contains M432V compared to NP_937884 (NM_198241.2); however the nucleotide change resulting in the amino acid change is more conserved between species, and it is located between functional domains of eIF4G1 (PABP-binding site and eIF4E-binding site), and does not affect its function as a translational factor Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:25 5
pEGFP-HDAC4
 
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1+ mentions
RRID:Addgene_45636 HDAC4 Homo sapiens Kanamycin PMID:17873280 Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:25 4
pD40-His/V5-c-Myc
 
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1+ mentions
RRID:Addgene_45597 c-Myc Mus musculus Ampicillin PMID:15048125 Alternate plasmid names: pD40-His-c-Myc; pcDNA-DEST40 Myc wt This His6-tagged c-Myc plasmid was created by PCR amplification of the coding sequence for wild-type murine c-Myc2 from the CMVMyc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-Myc. For plasmid usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971). Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:25 9
pET28b-Ava3855
 
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1+ mentions
RRID:Addgene_45743 Ava3855 Anabaena variabilis Kanamycin PMID:20813918 Backbone Marker:Novagen; Backbone Size:5293; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:26 1
pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP
 
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1+ mentions
RRID:Addgene_45574 co I-Sce I Ampicillin PMID:24121685 Backbone Size:5071; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin co indicates codon optimization for mammalian expression 2026-08-15 01:15:25 4
pCVL.Active/Repressed TLR (Sce)
 
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1+ mentions
RRID:Addgene_45575 eGFP with I-Sce I TS Homo sapiens Ampicillin PMID:24121685 Backbone Size:4483; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin embedded I-Sce I TS from 163-185 2026-08-15 01:15:25 1

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