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Species: Danio rerio
Genetic Insert: Cavin4b (PRDmt)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176017 Copy
Species: Danio rerio
Genetic Insert: Cavin4a
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176010 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176008 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:SITS-mCherry-ccdb (Addgene 118629); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176024 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:SITS-EGFP-ccdb (Addgene 118628); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176020 Copy
Species: Danio rerio
Genetic Insert: mpeg1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:Tol2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28972134
Proper citation: RRID:Addgene_166977 Copy
Species: Danio rerio
Genetic Insert: Csf3b
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Vertebrate expression (zebrafish, chicken, xenopus); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24128862
Proper citation: RRID:Addgene_168202 Copy
Species: Danio rerio
Genetic Insert: cdk2 sgRNAs
Vector Backbone Description: Vector Backbone:R4R3 pDest; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168250 Copy
Species: Danio rerio
Genetic Insert: sp5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11641225
Comments: Zebrafish sp5 (bts1) cDNA Full-length in pBluescript SK, cloned into EcoRI / XhoI. Useful for making antisense probe. Is the paralog of sp5-like (sp5l, clone 27-i2 from the Wnt8 upregulated microarray screen). The clone comes from library screening (library from Bruce Appel) and the library was constructed using EcoRI and XhoI linkers; The library was made into the Uni-ZAP XR Lambda vector, and from it Bluescript SK was excised.
Proper citation: RRID:Addgene_16884 Copy
Species: Danio rerio
Genetic Insert: 23f17
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15797017
Comments: Clone 23f17 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Noncoding, repetitive transcript. Similar and overlapping with many other regions in the genome. For antisense probe synthesis.
Expressed in paraxial and ventral mesoderm during gastrulation. Later in specific brain regions.
For antisense: EcoRI, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
Proper citation: RRID:Addgene_16883 Copy
Species: Danio rerio
Genetic Insert: axin2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15797017
Comments: Zebrafish axin2 cDNA (unclear if full-length or not) in pSPORT1. Clone 24h4 from microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library). For antisense probe synthesis.
Pretty ubiquitously expressed during gastrulation. Positively regulated by Wnt signaling.
For antisense: Asp718, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
Proper citation: RRID:Addgene_16882 Copy
Species: Danio rerio
Genetic Insert: wnt8 5'orf2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: 588 bp PCR product ligated into BamHI and EcoRI cut pSK+.
For probe: cut with BamHI, use T7 RNA polymerase.
Proper citation: RRID:Addgene_16898 Copy
Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Full length prickle-1 with 42bp of 5' UTR, cloned into pGEM-Teasy. The construct makes good in situ probes but does not translate in vitro due to an atg in the polylinker.
To make antisense probe: cut with Nco1, transcribe with SP6. For the sense strand cut with Nde1 and transcribe with T7.
Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_16897 Copy
Species: Danio rerio
Genetic Insert: Prickle-2
Vector Backbone Description: Backbone Size:0; Vector Backbone:CS2P+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Partial length prickle-2, contains the entire protein except the last 75 amino acids (predicted). Cloned into the BamH1 and Cla1 sites of CS2P+.
To make antisense probe: cut with BamH1, transcribe with T7. For the sense strand cut with Cla1 and transcribe with SP6.
Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_16896 Copy
Species: Danio rerio
Genetic Insert: Hrasls
Vector Backbone Description: Vector Backbone:Tol2-aACry; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_168772 Copy
Species: Danio rerio
Genetic Insert: E1b:eGFP
Vector Backbone Description: Backbone Size:3873; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: A. Emelyanov and S. Parinov. Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Developmental Biology, 320(1):113– 121, 2008. ISSN 00121606. doi: 10.1016/j.ydbio.2008.04.042.
A. Emelyanov, Y. Gao, N. I. Naqvi, and S. Parinov. Trans-kingdom transposition of the maize Dissociation element. Genetics, 174(3):1095–1104, 2006. ISSN 00166731. doi: 10.1534/genetics.106.061184.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_102417 Copy
Species: Danio rerio
Genetic Insert: myelin basic protein
Vector Backbone Description: Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12112375
Proper citation: RRID:Addgene_103007 Copy
Species: Danio rerio
Genetic Insert: kcnj2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173127 Copy
Species: Danio rerio
Genetic Insert: kcnj1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173126 Copy
Species: Danio rerio
Genetic Insert: kcnj15
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173145 Copy
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