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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pTK_FoxD3_enh_5_Citrine Resource Report Resource Website |
RRID:Addgene_130580 | FoxD3_enh_5 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Citrine; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:09 | 0 | ||
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pTK_Msx1_enh_389_Cerulean Resource Report Resource Website |
RRID:Addgene_130611 | Msx1_enh_389 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Cerulean; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:09 | 0 | ||
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pTK_Brn3_enh_250_Citrine Resource Report Resource Website |
RRID:Addgene_130612 | Brn3_enh_250 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Citrine; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:11 | 0 | ||
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pTK_Msx1_enh_264_Citrine Resource Report Resource Website |
RRID:Addgene_130610 | Msx1_enh_264 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Citrine; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:09 | 0 | ||
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pTK_Pax7_enh_368_Citrine Resource Report Resource Website |
RRID:Addgene_130608 | Pax7_enh_368 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Citrine; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:09 | 0 | ||
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pTK_Ets1_enh_117_Citrine Resource Report Resource Website |
RRID:Addgene_130609 | Ets1_enh_117 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Citrine; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:09 | 0 | ||
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pTK_Pax7_enh_143_Citrine Resource Report Resource Website |
RRID:Addgene_130602 | Pax7_enh_143 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Citrine; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:09 | 0 | ||
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pTK_Pax7_enh_194_Cerulean Resource Report Resource Website |
RRID:Addgene_130603 | Pax7_enh_194 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Cerulean; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:09 | 0 | ||
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pTK_Pax6_enh_2_Cerulean Resource Report Resource Website |
RRID:Addgene_130620 | Pax6_enh_2 | Gallus gallus | Ampicillin | PMID:31639368 | Backbone Size:4670; Vector Backbone:pTK_BsmBI_Cerulean; Vector Types:enhancer reporter; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:09 | 0 | ||
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pLVX-puro-cOVA-IRES-BFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_135074 | cOVA-IRES-BFP | Gallus gallus | Ampicillin | Backbone Size:8585; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:50 | 5 | |||
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CAP Z alpha 1 beta 1 CP Resource Report Resource Website 1+ mentions |
RRID:Addgene_13451 | capping protein alpha 1 beta 1 (chicken) | Gallus gallus | Ampicillin | PMID:9742448 | Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:05:45 | 3 | |
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M19 pCS-twhh-dsl-1myc Resource Report Resource Website |
RRID:Addgene_17156 | dorsalin-1 | Gallus gallus | Ampicillin | PMID:9314535 | Plasmid History: To place dsl-1myc after the twhh promoter, the DNA insert of dsl-1myc was first subcloned into the EcoRI site of expression vector pCS2+, which has a cytomegalovirus promoter and a polyadenylation site. The resulting plasmids were named pCS2+-dsl-1myc. To link dsl-1myc to the twhh promoter, the dsl-1myc insert was released from pCS2+-dsl-1myc by BamHI and XhoI digestion and then subcloned into pCS-twhh-beta -gal-vec BamHI and XhoI sites by replacing the beta -gal sequence. The final construct, pCS-twhh-dsl-1myc, contains the 5.2-kb twhh promoter and dsl-1myc. Notes for Use: Target dorsalin-1 expression in zebrafish notochord. Citation: Du et al., 1997, J. Cell Biology. | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:18 | 0 | |
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pBabe_Hyg_cOVA_T2A_mStrawberry Resource Report Resource Website |
RRID:Addgene_161737 | cytoplasmic ovalbumin | Gallus gallus | Ampicillin | PMID:32376951 | Please note Addgene NGS found a few discrepancies in the gag (truncated) region but depositor has confirmed this does not affect plasmid function. Depositor has verified plasmid's capacity to over-express the expected gene in target cells. | Backbone Marker:Addgene #1765; Vector Backbone:pBabe-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:08:57 | 0 | |
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pBabe_Zeo_cOVA_T2A_mStrawberry Resource Report Resource Website |
RRID:Addgene_161736 | cytoplasmic ovalbumin | Gallus gallus | Ampicillin | PMID:32376951 | Backbone Marker:Addgene #1766; Vector Backbone:pBabe-zeo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:08:57 | 0 | ||
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pBS chick BMP-4 [TJ#221] Resource Report Resource Website |
RRID:Addgene_16285 | BMP4 | Gallus gallus | Ampicillin | PMID:7553857 | To make an antisense probe, cut with BamHI and transcribe with T3. | Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:05 | 0 | |
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Tol2-GgVCL/*TBM Resource Report Resource Website |
RRID:Addgene_162790 | Mutated Gallus gallus vinculin (G454V) | Gallus gallus | Ampicillin | The incorporation of GgVCL and GgVCL/*TBM in a eukaryotic transient expression vector was done according to the following steps: 1) amplification of VCL sequence with Phusion-HF DNA pol and primers with a Kozac initiation sequence or RBS and side restriction enzyme sites (KpnI -FW and EcoRI –rev; ch-VCL custom primers); 2) extension with Taq DNA pol to generate A-ends; 3) Ligation to the T-ended vector TOPO TA #450640 through the topo cloning reaction and dilution in TE (Invitrogen 8019005?); 4) transformation of DH5α (C2987I) and selection with kanamycin and IPTG/Xgal (EU 0012-D); 5) Minipreps (740588.250) from presumed 3 TOPO-WT and 3 TOPO-MUT colonies, DNA quantification and diagnostic digestions with AccI, EcoRI or KpnI; 6) electrophoresis of abundant EcoRI + KpnI digestion products and band cutting; 7) purification of VCL sequence from gel and of EcoRI + KpnI digested and alkaline phosphatase (#EF0651) -treated pME-MCS (#237) with Macherey-Nagel #740609.250 kit; 8) DNA quantification; 9) ligation of each VCL sequence to pME-MCS plasmid using Ligase T4 (NEB MO202T); 10) transformation of DH5α (NEB C2987I) with the respective ligation reactions; minipreps (740588.250) to obtain pME-VCL, DNA quantification and diagnostic digestions with AccI , (KpnI-HF + EcoRI-HF) and BamHI-HF; 11) Tol2 kit LR reaction (Kwan et al 2007, DOI: 10.1002/dvdy.21343,) . Although the kit is originally aimed at zebrafish, it had already been assayed in mammalian cells. The LR reaction involved the use of Gateway LR Clonase II Plus Enzyme Mix (Invitrogen 12538-120) to fuse 4 plasmids in one: the 5’entry plasmid with a β-actin promoter called p5E-actin (#299), the middle entry plasmid with the VCL insert pME-VCL (wt or mut), the 3’entry plasmid p3E-IRES-nls-GFP (#391) and the destination vector pDestTol2pA2 (#394). The expected LR reaction product is a 14,5 KB eukaryotic expression vector which can be amplified in bacteria. NEB C3019I bacteria were transformed with the LR reaction product. Three ampicillin-resistant clear colonies of each (β-actin- wt or mutVCLIRES nclGFP) were chosen and amplified. Minipreps, DNA quantification and diagnostic digestions with (KpnI+AccI) and Pvu-IIHF were carried. Finally, 200 mL of liquid culture were used to prepare MIDIPREPS (XXX). About 180 μg good quality DNA of each (Tol2-GgVCL or Tol2- GgVCL/*TBM) expression vectors were obtained and checked by digestion with restriction enzymes. | Vector Backbone:destination vector pDestTol2pA2(#394); Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin | changed Gly 454 to Val | 2026-08-15 01:09:05 | 0 | |
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pBS chick PEA3 [TJ#51] Resource Report Resource Website |
RRID:Addgene_16279 | PEA3 | Gallus gallus | Ampicillin | PMID:9814709 | To make an antisense probe, cut with NotI and transcribe with T7. | Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:05 | 0 | |
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Tol2-GgVCL/WT Resource Report Resource Website |
RRID:Addgene_162787 | Gallus gallus vinculin | Gallus gallus | Ampicillin | The incorporation of GgVCL and GgVCL/*TBM in a eukaryotic transient expression vector was done according to the following steps: 1) amplification of VCL sequence with Phusion-HF DNA pol and primers with a Kozac initiation sequence or RBS and side restriction enzyme sites (KpnI -FW and EcoRI –rev; ch-VCL custom primers); 2) extension with Taq DNA pol to generate A-ends; 3) Ligation to the T-ended vector TOPO TA #450640 through the topo cloning reaction and dilution in TE (Invitrogen 8019005?); 4) transformation of DH5α (C2987I) and selection with kanamycin and IPTG/Xgal (EU 0012-D); 5) Minipreps (740588.250) from presumed 3 TOPO-WT and 3 TOPO-MUT colonies, DNA quantification and diagnostic digestions with AccI, EcoRI or KpnI; 6) electrophoresis of abundant EcoRI + KpnI digestion products and band cutting; 7) purification of VCL sequence from gel and of EcoRI + KpnI digested and alkaline phosphatase (#EF0651) -treated pME-MCS (#237) with Macherey-Nagel #740609.250 kit; 8) DNA quantification; 9) ligation of each VCL sequence to pME-MCS plasmid using Ligase T4 (NEB MO202T); 10) transformation of DH5α (NEB C2987I) with the respective ligation reactions; minipreps (740588.250) to obtain pME-VCL, DNA quantification and diagnostic digestions with AccI , (KpnI-HF + EcoRI-HF) and BamHI-HF; 11) Tol2 kit LR reaction (Kwan et al 2007, DOI: 10.1002/dvdy.21343, ). Although the kit is originally aimed at zebrafish, it had already been assayed in mammalian cells. The LR reaction involved the use of Gateway LR Clonase II Plus Enzyme Mix (Invitrogen 12538-120) to fuse 4 plasmids in one: the 5’entry plasmid with a β-actin promoter called p5E-actin (#299), the middle entry plasmid with the VCL insert pME-VCL (wt or mut), the 3’entry plasmid p3E-IRES-nls-GFP (#391) and the destination vector pDestTol2pA2 (#394). The expected LR reaction product is a 14,5 KB eukaryotic expression vector which can be amplified in bacteria. NEB C3019I bacteria were transformed with the LR reaction product. Three ampicillin-resistant clear colonies of each (β-actin- wt or mutVCL- IRES nclGFP) were chosen and amplified. Minipreps, DNA quantification and diagnostic digestions with (KpnI+AccI) and Pvu-IIHF were carried. Finally, 200 mL of liquid culture were used to prepare MIDIPREPS (XXX). About 180 µg good quality DNA of each (Tol2-GgVCL or Tol2-GgVCL/*TBM) expression vectors were obtained and checked by digestion with restriction enzymes. | Vector Backbone:destination vector pDestTol2pA2(#394); Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:09:05 | 0 | |
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pDDGFP-Leu2d_GgPCFT Resource Report Resource Website |
RRID:Addgene_165414 | GgPCFT | Gallus gallus | Ampicillin | PMID:34040256 | Backbone Size:8654; Vector Backbone:pDDGFP_LEU2d; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:28 | 0 | ||
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pet 3d double tentacle delete CP Resource Report Resource Website |
RRID:Addgene_13301 | capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids) | Gallus gallus | Ampicillin | PMID:12956956 | Soeno et al., 1998 | Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Capping protein alpha 1 (with 28 amino acids deleted in the C-terminus) and beta 1 (with 34 amino acids deleted in the C-terminus) | 2026-08-15 01:05:32 | 0 |
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