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Species: Synthetic
Genetic Insert: v5.9
Vector Backbone Description: Backbone Marker:modified from pASK-IBA37plus (IBA GmbH); Vector Backbone:pAtetO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29629759
Proper citation: RRID:Addgene_155259 Copy
Species: Synthetic
Genetic Insert: Rfx Cas13d CRISPR RNA BFP
Vector Backbone Description: Backbone Size:1807; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353252
Comments: Plasmid derived from Addgene #109054. Clone new guides by digesting with BbsI then annealing, ligating oligos with desired spacer.
Proper citation: RRID:Addgene_155306 Copy
Species: Synthetic
Genetic Insert: PfAMA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32888502
Comments: Please note: Plasmid contains N162K, T288V, S373D, N422D, S423K, and N499Q mutations in AMA1 compared to the NCBI reference sequence P50490.1. It is not known how these mutations affect plasmid function.
Proper citation: RRID:Addgene_155308 Copy
Species: Synthetic
Genetic Insert: PfEBA175
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32888502
Proper citation: RRID:Addgene_155309 Copy
Species: Synthetic
Genetic Insert: Superfolder GFP fused with SARS-CoV-2 fluorescent reporter 2
Vector Backbone Description: Backbone Size:8965; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353252
Comments: Please note: The plasmid contains a single A nucleotide deletion in the SARS-CoV-2 N gene ORF that causes a frameshift and early termination. This change is not expected to affect plasmid function as the only ORF on the plasmid is sfGFP (the RdRP and N gene fragments occur after the stop codon and are in the 3'UTR of sfGFP).
Proper citation: RRID:Addgene_155304 Copy
Species: Synthetic
Genetic Insert: 4E1H.95_LUMABS
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33398169
Proper citation: RRID:Addgene_155208 Copy
Species: Synthetic
Genetic Insert: 4E2H.210_LUMABS
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33398169
Proper citation: RRID:Addgene_155209 Copy
Species: Synthetic
Genetic Insert: 4E2H.210
Vector Backbone Description: Vector Backbone:pET11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33398169
Proper citation: RRID:Addgene_155212 Copy
Species: Synthetic
Genetic Insert: iRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155229 Copy
Species: Synthetic
Genetic Insert: H11-H4
Vector Backbone Description: Vector Backbone:pOPINO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32661423
Proper citation: RRID:Addgene_155364 Copy
Species: Synthetic
Genetic Insert: Nb H11-D4
Vector Backbone Description: Vector Backbone:pOPINO; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32661423
Proper citation: RRID:Addgene_155363 Copy
Species: Synthetic
Genetic Insert: PphlF-TA4 in dCas12a oscillator crRNAs
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155385 Copy
Species: Synthetic
Genetic Insert: dCas12a oscillator crRNAs
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32609820
Proper citation: RRID:Addgene_155381 Copy
Species: Synthetic
Genetic Insert: PfEBA175
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32888502
Proper citation: RRID:Addgene_155312 Copy
Species: Synthetic
Genetic Insert: humanized EBpaRS/BstYam
Vector Backbone Description: Backbone Marker:Clontech, major modifications; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929862
Comments: The plasmid has been developed for efficient incorporation of Bpa in mammalian cells, first reported in Böttke et al, EMBO reports, 2020. With respect to similar plasmids from other labs, this plasmid contains a humanized gene for EBpaRS and 4 copies of the U6-BstYam tRNA cassette downstream of the synthetase cassette.
Proper citation: RRID:Addgene_155342 Copy
Species: Synthetic
Genetic Insert: mCerulean
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156376 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156379 Copy
Species: Synthetic
Genetic Insert: ermCL-sfGFP
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156373 Copy
Species: Synthetic
Genetic Insert: mTagBFP
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156375 Copy
Species: Synthetic
Genetic Insert: Sirius
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156374 Copy
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