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Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172089 Copy
Species: Synthetic
Genetic Insert: EBFP, tTAn-InteinN
Vector Backbone Description: Backbone Size:5321; Vector Backbone:pAAV-EF1a-DIO-TVA950-T2A-CVS12G-WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35739125
Comments: Used Infusion cloning. 5' cloning site: XbaI (not destroyed), 3' cloning site: EcoRV (not destroyed). Please visit https://www.biorxiv.org/content/10.1101/2021.04.05.438407v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172122 Copy
Species: Synthetic
Genetic Insert: InteinC-rtTAc
Vector Backbone Description: Backbone Size:5321; Vector Backbone:pAAV-EF1a-DIO-TVA950-T2A-CVS11G-WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35739125
Comments: Used Infusion cloning. 5' cloning site: XbaI (not destroyed), 3' cloning site: EcoRV (not destroyed). Please visit https://www.biorxiv.org/content/10.1101/2021.04.05.438407v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172121 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172087 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172086 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172085 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172084 Copy
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172156 Copy
Species: Synthetic
Genetic Insert: Para (pylBCD 36A_sub-pop1); AraC
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172155 Copy
Species: Synthetic
Genetic Insert: Para (Sumo-tagged pylBCD); AraC
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172154 Copy
Species: Synthetic
Genetic Insert: Para sfGFP.2TAG; Plpp pylS; PproK pylT; AraC
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172153 Copy
Species: Danio rerio
Genetic Insert: Sp5l promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon.
(as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 clone.
upstream of firefly luciferase in the pGL3 Basic promoter. 6 of the putative Tcf/Lef sites mutated.
(all introduce additional restriction sites); cloned by PCR-based in vitro synthesis from 28 overlapping.
oligos. cloned NdeI / HindIII to exchange wild-type fragment in pGL3 Sp5l P1203.
Species of Sequence Origin: zebrafish.
Proper citation: RRID:Addgene_17209 Copy
Species: Synthetic
Genetic Insert: Para (pylBCD 3f2+C); AraC
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172165 Copy
Species: Synthetic
Genetic Insert: Para (pylBCD 3f2v2+B); AraC
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172164 Copy
Species: Homo sapiens
Genetic Insert: CD63
Vector Backbone Description: Backbone Size:9331; Vector Backbone:pLenti-CMV Blast DEST (706-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32350252
Proper citation: RRID:Addgene_172117 Copy
Species: Homo sapiens
Genetic Insert: hNGN2
Vector Backbone Description: Backbone Size:13245; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36459969
Comments: This plasmid has been found to run as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_172115 Copy
Species: N.Neisseria meningitidis serogroup B (strain MC58)
Genetic Insert: MetN
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5245; Vector Backbone:pET21b+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34409939
Proper citation: RRID:Addgene_172114 Copy
Species: Homo sapiens
Genetic Insert: hNGN2
Vector Backbone Description: Backbone Size:12000; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36459969
Proper citation: RRID:Addgene_172113 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172078 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172077 Copy
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