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Species: Synthetic
Genetic Insert: cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
Vector Backbone Description: Vector Backbone:pAYCU268; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30867327
Comments: The plasmid contains the following relevant inserts: 1. C. albicans Autonomously Replicating Sequence 2 for episomal propagation in C. tropicalis; 2. CAS9 sequence expressed from a Meyerozyma guilliermondii promoter; 3. Cassette for the expression of the sgRNA from a Ashbya gossypii RNA pol II promoter. The mature sgRNA is released following RNase Z processing of the upstream tRNA and self-cleavage of the dowstream HDV Ribozyme. The guide targeting the gene of interest can be cloned into the SapI/BspQI digested plasmid. The regulatory elements (species of origin of promoters/terminators) are described in the map.
Proper citation: RRID:Addgene_133813 Copy
Species: Synthetic
Genetic Insert: human codon optimized Eriv-AcrIIA1
Vector Backbone Description: Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32325050
Proper citation: RRID:Addgene_133800 Copy
Species: Synthetic
Genetic Insert: U6 promoter
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_133961 Copy
Species: Synthetic
Genetic Insert: sfGFP(Sp)
Vector Backbone Description: Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29195004
Proper citation: RRID:Addgene_133833 Copy
Species: Synthetic
Genetic Insert: mKate(KPS12)
Vector Backbone Description: Backbone Marker:Dunn and Handelsman, 1999; Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29195004
Proper citation: RRID:Addgene_133831 Copy
Species: Synthetic
Genetic Insert: AtzO-sfGFP
Vector Backbone Description: Backbone Size:3096; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32078765
Comments: A154T mutation in superfolder GFP does not impact plasmid function
Proper citation: RRID:Addgene_133872 Copy
Species: Synthetic
Genetic Insert: sfGFP(SPS6)
Vector Backbone Description: Backbone Marker:Dunn and Handelsman, 1999; Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29195004
Proper citation: RRID:Addgene_133906 Copy
Species: Synthetic
Genetic Insert: Citron1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32875085
Proper citation: RRID:Addgene_134300 Copy
Species: Synthetic
Genetic Insert: Citroff1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32875085
Proper citation: RRID:Addgene_134301 Copy
Species: Synthetic
Genetic Insert: Citroff1 S245A
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32875085
Proper citation: RRID:Addgene_134302 Copy
Species: Synthetic
Genetic Insert: kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31672972
Comments: pSiMPlk_N should be cotransformed with pSiMPlk_C (or vice versa) in E. coli to get a reconstituted functional APT thereby conferring resistance against kanamycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlk_N + pSiMPlk_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134310 Copy
Species: Synthetic
Genetic Insert: cmR_gp41-1 N-intein + cmR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31672972
Comments: pSiMPlc_N should be cotransformed with pSiMPlc_C (or vice versa) in E. Coli to get a reconstituted functional CAT thereby conferring resistance against chloramphenicol. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlc_N + pSiMPlc_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134312 Copy
Species: Synthetic
Genetic Insert: A1
Vector Backbone Description: Vector Backbone:pFA6a-natMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134428 Copy
Species: Synthetic
Genetic Insert: A1-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-hphMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134429 Copy
Species: Synthetic
Genetic Insert: S6
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134434 Copy
Species: Synthetic
Genetic Insert: S6
Vector Backbone Description: Vector Backbone:pFA6a-natMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134436 Copy
Species: Synthetic
Genetic Insert: S6-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-hphMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134437 Copy
Species: Synthetic
Genetic Insert: S6-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-kanMX6; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134438 Copy
Species: Synthetic
Genetic Insert: SRS
Vector Backbone Description: Vector Backbone:pFA6a-natMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134444 Copy
Species: Synthetic
Genetic Insert: SRS-FLAG
Vector Backbone Description: Vector Backbone:pFA6a-hphMX4; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32401365
Proper citation: RRID:Addgene_134445 Copy
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