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Species: Other
Genetic Insert: Tth DNA polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C); can therefore be used for PCR amplification of DNA fragments. Also accepts modified deoxyribonucleotides as substrates and can thus be used to label DNA fragments with radiolabeled nucleotides, digoxigenin, or biotin. Intrinsic Mn-dependent RT activity allows the enzyme to efficiently transcribe RNA targets into cDNA. Tth DNA Polymerase has been used for real time PCR.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165533 Copy
Species: Other
Genetic Insert: Bst HF
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Bst-HF is a high-fidelity strand displacing polymerase that is able to overcome complex/problematic sequences, allowing amplification of large fragments and DNA sequences with over 80% GC content. Bst-HF has an error rate of about 7.8x10^-7, a fidelity about 20 times higher than standard Bst, and better than Phusion High-Fidelity DNA Polymerase in High-GC buffer.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165539 Copy
Species: Other
Genetic Insert: M.PstI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Methylase that recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165536 Copy
Species: Other
Genetic Insert: SapI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165537 Copy
Species: Other
Genetic Insert: HCoV-HKU1 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166014 Copy
Species: Other
Genetic Insert: HCoV-NL63 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166017 Copy
Species: Other
Genetic Insert: SARS-CoV-2 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166011 Copy
Species: Other
Genetic Insert: GPI anchored TurboID
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177716
Proper citation: RRID:Addgene_166055 Copy
Species: Other
Genetic Insert: mEGFP
Vector Backbone Description: Vector Backbone:pet22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166439 Copy
Species: Other
Genetic Insert: Spaghetti monster Myc
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166239 Copy
Species: Other
Genetic Insert: Spaghetti monster HA
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166238 Copy
Species: Other
Genetic Insert: mCardinal
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166236 Copy
Species: Other
Genetic Insert: I-sceI, sacB
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32448155
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_166651 Copy
Species: Other
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pTargetF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33764372
Proper citation: RRID:Addgene_166581 Copy
Species: Other
Genetic Insert: REP/CAP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31788496
Proper citation: RRID:Addgene_166921 Copy
Species: Other
Genetic Insert: A3A-Cas9-UDG-T2A-AP lyase
Vector Backbone Description: Vector Backbone:pHUE411; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32601432
Proper citation: RRID:Addgene_167361 Copy
Species: Other
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Comments: Gene was obtained from collaborator Andor Kiss, Miami University of Ohio.
Proper citation: RRID:Addgene_46167 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET-28b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24873830
Comments: Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis.
This plasmid was used to express a His-tagged Cas9 protein in E. coli and purify it by nickel-based affinity purification.The Cas9 protein and sgRNA were incubated at room temperature to form the Cas9 protein/sgRNA complex in vitro, which was then microinjected into zebrafish zygotes. See publication for more details.
Proper citation: RRID:Addgene_47327 Copy
Species: Other
Genetic Insert: minCMV-dTomato_pA
Vector Backbone Description: Vector Backbone:pcrBluntII-Topo; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47320 Copy
Species: Other
Genetic Insert: Cas9m4-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Comments: This plasmid contains a human codon optimized form of cas9.
Proper citation: RRID:Addgene_47319 Copy
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