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Species: Mus musculus
Genetic Insert: MEKK3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14634666
Proper citation: RRID:Addgene_12189 Copy
Species: Nostoc punctiforme
Genetic Insert: Oth-NpuDnaBΔ290 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3981; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31665813
Proper citation: RRID:Addgene_121914 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6472; Vector Backbone:pETM-NG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: ccdB Survival cells are NOT suitable for the unmodified plasmid containing the ccdB gene. The plasmid is provided in the recommended DB3.1 strain. Please see supplemental file for LP1 and LP2 primers to use for SLIC cloning. A detailed protocol for using pCoofy plasmids is available at https://p4eu.org/wiki .
Use one of the following linearization primer pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer:
LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3'
LP2 reverse vector primer: SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3'
Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_122013 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Choline kinase
Vector Backbone Description: Backbone Marker:Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D.; Backbone Size:3465; Vector Backbone:pSEVA228; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30584096
Proper citation: RRID:Addgene_122018 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5917; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: ccdB Survival cells are NOT suitable for the unmodified plasmid containing the ccdB gene. The plasmid is provided in the recommended DB3.1 strain. Please see supplemental file for LP1 and LP2 primers to use for SLIC cloning. A detailed protocol for using pCoofy plasmids is available at https://p4eu.org/wiki .
Use one of the following linearization primer pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer:
LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3'
LP2 reverse vector primer: SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3'
Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_122006 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: intergenic terminator from yeast IMD2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5900; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30616008
Proper citation: RRID:Addgene_122061 Copy
Species: Homo sapiens
Genetic Insert: CYFIP1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmcherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24667445
Proper citation: RRID:Addgene_122051 Copy
Genetic Insert: none
Vector Backbone Description: Backbone Size:6073; Vector Backbone:pUC57 and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31548010
Proper citation: RRID:Addgene_121999 Copy
Species: Danio rerio
Genetic Insert: mfn2 gRNA
Vector Backbone Description: Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32788232
Proper citation: RRID:Addgene_121994 Copy
Species: Danio rerio
Genetic Insert: opa1 gRNA
Vector Backbone Description: Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32788232
Proper citation: RRID:Addgene_121995 Copy
Vector Backbone Description: Backbone Marker:Esteban Martínez-García and Víctor de Lorenzo; Backbone Size:3168; Vector Backbone:pEMG; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122086 Copy
Species: Mus musculus
Genetic Insert: Myocardin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pShuttle; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Comments: Myocardin contains an A435T difference compared to the Myocd NCBI entry. The functional consequence of this difference is unknown.
Proper citation: RRID:Addgene_122122 Copy
Species: Homo sapiens
Genetic Insert: Brg1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Proper citation: RRID:Addgene_122115 Copy
Species: Homo sapiens
Genetic Insert: Brg1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Proper citation: RRID:Addgene_122117 Copy
Genetic Insert: 10xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122170 Copy
Genetic Insert: 12xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122172 Copy
Species: Homo sapiens
Genetic Insert: His6 MBP TEV HDAC8_374
Vector Backbone Description: Backbone Marker:Scott Gradia; Backbone Size:6465; Vector Backbone:pET His6 MBP TEV LIC cloning vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28846375
Proper citation: RRID:Addgene_122174 Copy
Species: Aequorea victoria
Genetic Insert: RS-GFP
Vector Backbone Description: Backbone Marker:prof. Pascal Ratet (French National Centre for Scientific Research); Backbone Size:12400; Vector Backbone:pCP60 (sequence not available in databases) - derivative of pBIN19; Vector Types:Plant Expression, binary vector for Escherichia coli and Agrobacterium tumefaciens-mediated plant transformation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19386122
Proper citation: RRID:Addgene_122173 Copy
Genetic Insert: 2xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122162 Copy
Genetic Insert: 7xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122167 Copy
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