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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pOpen-TthpolA Resource Report Resource Website |
RRID:Addgene_165533 | Tth DNA polymerase | Other | Ampicillin | DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C); can therefore be used for PCR amplification of DNA fragments. Also accepts modified deoxyribonucleotides as substrates and can thus be used to label DNA fragments with radiolabeled nucleotides, digoxigenin, or biotin. Intrinsic Mn-dependent RT activity allows the enzyme to efficiently transcribe RNA targets into cDNA. Tth DNA Polymerase has been used for real time PCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-Bst HF Resource Report Resource Website |
RRID:Addgene_165539 | Bst HF | Other | Ampicillin | Bst-HF is a high-fidelity strand displacing polymerase that is able to overcome complex/problematic sequences, allowing amplification of large fragments and DNA sequences with over 80% GC content. Bst-HF has an error rate of about 7.8x10^-7, a fidelity about 20 times higher than standard Bst, and better than Phusion High-Fidelity DNA Polymerase in High-GC buffer. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-PstIM Resource Report Resource Website |
RRID:Addgene_165536 | M.PstI | Other | Ampicillin | Methylase that recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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pOpen-SapIR Resource Report Resource Website |
RRID:Addgene_165537 | SapI | Other | Ampicillin | Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
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R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166014 | HCoV-HKU1 S (spike) | Other | Ampicillin | PMID:32504802 | DNA sequence optimized for mammalian expression | Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:33 | 2 | |
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R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166017 | HCoV-NL63 S (spike) | Other | Ampicillin | PMID:32504802 | DNA sequence optimized for mammalian expression | Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:33 | 1 | |
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R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G Resource Report Resource Website 1+ mentions |
RRID:Addgene_166011 | SARS-CoV-2 S (spike) | Other | Ampicillin | PMID:32504802 | DNA sequence optimized for mammalian expression | Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D614G | 2026-08-15 01:09:33 | 2 |
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pZac2.1-GfaABC1D-TurboID(full)-HA-GPI Resource Report Resource Website 1+ mentions |
RRID:Addgene_166055 | GPI anchored TurboID | Other | Ampicillin | PMID:33177716 | Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:33 | 1 | ||
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Pet22b-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_166439 | mEGFP | Other | Ampicillin | PMID:33606996 | Vector Backbone:pet22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:35 | 2 | ||
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pSL24b-Linker-smMyc-FP-DHFR-LoxP Resource Report Resource Website |
RRID:Addgene_166239 | Spaghetti monster Myc | Other | Ampicillin | Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:09:34 | 0 | ||
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pSL24a-Linker-smHA-FP-DHFR-LoxP Resource Report Resource Website |
RRID:Addgene_166238 | Spaghetti monster HA | Other | Ampicillin | Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:09:35 | 0 | ||
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pTUBmCardinalSagCAT.dna Resource Report Resource Website |
RRID:Addgene_166236 | mCardinal | Other | Ampicillin | Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:09:34 | 0 | ||
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pFOK Resource Report Resource Website 1+ mentions |
RRID:Addgene_166651 | I-sceI, sacB | Other | Kanamycin | PMID:32448155 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Vector Backbone:R6K; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:37 | 1 | |
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pEcgRNA Resource Report Resource Website 1+ mentions |
RRID:Addgene_166581 | ccdB | Other | Spectinomycin | PMID:33764372 | Vector Backbone:pTargetF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:09:36 | 5 | ||
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AAV-F in pAR-9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_166921 | REP/CAP | Other | Ampicillin | PMID:31788496 | Vector Backbone:unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:39 | 3 | ||
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pH-AFID-3 Resource Report Resource Website |
RRID:Addgene_167361 | A3A-Cas9-UDG-T2A-AP lyase | Other | Kanamycin | PMID:32601432 | Vector Backbone:pHUE411; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | WT | 2026-08-15 01:09:43 | 0 | |
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Dissostichus mawsoni cryaa Resource Report Resource Website |
RRID:Addgene_46167 | Alpha A-crystallin | Other | Ampicillin | PMID:22479631 | Gene was obtained from collaborator Andor Kiss, Miami University of Ohio. | Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:29 | 0 | |
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pET-28b-Cas9-His Resource Report Resource Website 10+ mentions |
RRID:Addgene_47327 | Cas9 | Other | Kanamycin | PMID:24873830 | Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis. This plasmid was used to express a His-tagged Cas9 protein in E. coli and purify it by nickel-based affinity purification.The Cas9 protein and sgRNA were incubated at room temperature to form the Cas9 protein/sgRNA complex in vitro, which was then microinjected into zebrafish zygotes. See publication for more details. | Backbone Size:5368; Vector Backbone:pET-28b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:40 | 35 | |
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reporter-gT1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_47320 | minCMV-dTomato_pA | Other | Kanamycin | PMID:23907171 | Vector Backbone:pcrBluntII-Topo; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:41 | 7 | ||
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Cas9m4-VP64 Resource Report Resource Website 10+ mentions |
RRID:Addgene_47319 | Cas9m4-VP64 | Other | Ampicillin | PMID:23907171 | This plasmid contains a human codon optimized form of cas9. | Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:40 | 13 |
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