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Genetic Insert: 9xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122169 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122253 Copy
Species: Caenorhabditis elegans
Genetic Insert: mtagBFP2
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122255 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122254 Copy
Species: Synthetic
Genetic Insert: gRNAwcaF159
Vector Backbone Description: Vector Backbone:pBbE2k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30177768
Proper citation: RRID:Addgene_122259 Copy
Species: Homo sapiens
Genetic Insert: NPM-ALK fusion protein
Vector Backbone Description: Backbone Size:5289; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32193476
Proper citation: RRID:Addgene_122462 Copy
Genetic Insert: Strep-tagged 3xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122490 Copy
Genetic Insert: Strep-tagged 5xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122492 Copy
Genetic Insert: Strep-tagged 10xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122497 Copy
Genetic Insert: Strep-tagged 9xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122496 Copy
Genetic Insert: Strep-tagged 12xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122499 Copy
Genetic Insert: Strep-tagged 1xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122488 Copy
Genetic Insert: Strep-tagged 2xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122489 Copy
Species: Homo sapiens
Genetic Insert: tUI
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122661 Copy
Species: Homo sapiens
Genetic Insert: tISR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122664 Copy
Species: Homo sapiens
Genetic Insert: tIVR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122663 Copy
Species: Homo sapiens
Genetic Insert: ferritin heavy chain 1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_122652 Copy
Species: Listeria innocua
Genetic Insert: DPS, listeria
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14514305
Comments: This plasmid is also used in the following publications:
Resnick, D. A., Gilmore, K., Idzerda, Y. U., Klem, M. T., Allen, M., Douglas, T., … Young, M. (2006). Magnetic properties of Co 3O 4 nanoparticles mineralized in Listeria innocua Dps. Journal of Applied Physics, 99(8), 1–4. https://doi.org/10.1063/1.2163839
Okuda, M., Suzumoto, Y., Iwahori, K., Kang, S., Uchida, M., Douglas, T., & Yamashita, I. (2010). Bio-templated CdSe nanoparticle synthesis in a cage shaped protein, Listeria-Dps, and their two dimensional ordered array self-assembly. Chemical Communications, 46(46), 8797–8799. https://doi.org/10.1039/c0cc03298k
Usselman, R. J., Russek, S. E., Klem, M. T., Allen, M. A., Douglas, T., Young, M., … Singel, D. J. (2012). Temperature dependence of electron magnetic resonance spectra of iron oxide nanoparticles mineralized in Listeria innocua protein cages. Journal of Applied Physics, 112(8), 1–9. https://doi.org/10.1063/1.4757964
Proper citation: RRID:Addgene_122654 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens forkhead box P2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5269; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30887622
Proper citation: RRID:Addgene_122647 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens forkhead box P2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5269; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30887622
Proper citation: RRID:Addgene_122649 Copy
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