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Species: Synthetic
Genetic Insert: Nsp15
Vector Backbone Description: Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37704626
Comments: insert amino acid sequence: GLQSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLx
Proper citation: RRID:Addgene_156481 Copy
Species: Homo sapiens
Genetic Insert: TIF1 gamma
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Proper citation: RRID:Addgene_15734 Copy
Vector Backbone Description: Backbone Size:4816; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.111, Ligation number L3610.
Proper citation: RRID:Addgene_1575 Copy
Species: Homo sapiens
Genetic Insert: Fibroblast growth factor 2
Vector Backbone Description: Backbone Size:6377; Vector Backbone:pCAG-HA-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157663 Copy
Species: Mus musculus
Genetic Insert: mH2A.Z-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pIND; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15770 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp5
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157685 Copy
Species: Homo sapiens
Genetic Insert: hemicholinium-3-sensitive choline transporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11027560
Proper citation: RRID:Addgene_15766 Copy
Species: Homo sapiens
Genetic Insert: Jnk3a2
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8654373
Proper citation: RRID:Addgene_15748 Copy
Species: Homo sapiens
Genetic Insert: Jnk2a2
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8654373
Proper citation: RRID:Addgene_15744 Copy
Species: Synthetic
Genetic Insert: 12x601_30bp linker
Vector Backbone Description: Vector Backbone:pWM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Comments: occasional plasmid instability in dam-/dcm- strains
High copy plasmid (pUC-based, but medium yield)
Proper citation: RRID:Addgene_157789 Copy
Species: Synthetic
Genetic Insert: 12x601_25bp linker
Vector Backbone Description: Vector Backbone:pWM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Comments: occasional plasmid instability in dam-/dcm- strains
High copy plasmid (pUC-based, but medium yield)
Proper citation: RRID:Addgene_157788 Copy
Species: Synthetic
Genetic Insert: 12x601_20bp linker
Vector Backbone Description: Vector Backbone:pWM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Comments: occasional plasmid instability in dam-/dcm- strains
High copy plasmid (pUC-based, but medium yield)
Proper citation: RRID:Addgene_157787 Copy
Species: Homo sapiens
Genetic Insert: FMR1
Vector Backbone Description: Backbone Size:13450; Vector Backbone:Addgene #49535; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32179589
Proper citation: RRID:Addgene_157783 Copy
Species: Homo sapiens
Genetic Insert: Sec16L
Vector Backbone Description: Backbone Marker:Glick Lab; Backbone Size:4731; Vector Backbone:pmGFP-c1 ( modified from pEGFP-c1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17192411
Comments: Please note that we have identified a K466R missense mutation in the Sec16L/Sec16A region of this plasmid, and the start codon may not have been correctly identified when constructing this plasmid which may have resulted in the gene being truncated. However, we do not think that this should impact plasmid functionality.
Proper citation: RRID:Addgene_15776 Copy
Species: Homo sapiens
Genetic Insert: Sec16S
Vector Backbone Description: Backbone Marker:Glick Lab; Backbone Size:4731; Vector Backbone:pmGFPc1( modified from pEGFP-c1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17192411
Proper citation: RRID:Addgene_15775 Copy
Species: Homo sapiens
Genetic Insert: p300HAT
Vector Backbone Description: Vector Backbone:pETduet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Comments: ySIR2 required for expression & purification - A synthetic ORF encoding the histone deacetylase domain of S. cerevisiae protein SIR2 was amplified from a dsDNA synthesized by IDT using PCR and primers adding a 5'-proximal translation start codon and a 3'-proximal translation stop codon and XhoI restriction endonuclease recognition site. XhoI restriction endonuclease (NEB) digested PCR product encoding amino acids 87-562 of wild-type SIR2 was cloned into the pETduet-1 expression vector (Novagen) using a XhoI and a blunted NdeI restriction endonuclease digestion site (NEB).
Proper citation: RRID:Addgene_157793 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin conjugating enzyme UbE2B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4900; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17597759
Proper citation: RRID:Addgene_15781 Copy
Species: SARS-CoV-2
Genetic Insert: Nprot
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157728 Copy
Vector Backbone Description: Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral, CaTCH barcode cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32719478
Comments: mCMV_eGFP_EF1as_eBFP2
Proper citation: RRID:Addgene_157746 Copy
Species: Lymphocytic choriomeningitis virus (strain WE)
Genetic Insert: Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4831; Vector Backbone:pHMCV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15542136
Proper citation: RRID:Addgene_15793 Copy
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