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Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAftsI
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149590 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Comments: Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation.
Proper citation: RRID:Addgene_149588 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10TC); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149589 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149627 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149628 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149629 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAmreB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149654 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149655 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149658 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10AC2); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149660 Copy
Species: Synthetic
Genetic Insert: 14X miR-146a binding sites
Vector Backbone Description: Backbone Size:8518; Vector Backbone:pSCALPS_mCherry_GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32205878
Proper citation: RRID:Addgene_149718 Copy
Species: Synthetic
Genetic Insert: CBG99
Vector Backbone Description: Backbone Size:6261; Vector Backbone:pMSCV-puro; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160544
Comments: Please note: This plasmid contains a G255V mutation in CBG99. This mutation is not expected to affect plasmid function.
Proper citation: RRID:Addgene_139195 Copy
Species: Synthetic
Genetic Insert: CBRed
Vector Backbone Description: Backbone Size:6586; Vector Backbone:pMSCV-hygro; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160544
Proper citation: RRID:Addgene_139196 Copy
Species: Synthetic
Genetic Insert: EBFP2-P2A-Blasticidin S Deaminase
Vector Backbone Description: Vector Backbone:pPD005 pcDNA Golden Gate; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32034124
Comments: Leonard Lab plasmid reference number: L1506
Proper citation: RRID:Addgene_139233 Copy
Species: Synthetic
Genetic Insert: EBFP2-P2A-Blasticidin S Deaminase
Vector Backbone Description: Vector Backbone:pPD005 pcDNA Golden Gate; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32034124
Comments: Leonard Lab plasmid reference number: L1510
Proper citation: RRID:Addgene_139237 Copy
Species: Synthetic
Genetic Insert: Linker2
Vector Backbone Description: Vector Backbone:pPD005 pcDNA Golden Gate; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Leonard Lab plasmid reference number: L1561
Proper citation: RRID:Addgene_139240 Copy
Species: Synthetic
Genetic Insert: Linker6
Vector Backbone Description: Vector Backbone:pPD005 pcDNA Golden Gate; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Leonard Lab plasmid reference number: L1565
Proper citation: RRID:Addgene_139244 Copy
Species: Synthetic
Genetic Insert: Linker9
Vector Backbone Description: Vector Backbone:pPD005 pcDNA Golden Gate; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Leonard Lab plasmid reference number: L1568
Proper citation: RRID:Addgene_139247 Copy
Species: Synthetic
Genetic Insert: Linker8
Vector Backbone Description: Vector Backbone:pPD005 pcDNA Golden Gate; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Leonard Lab plasmid reference number: L1567
Proper citation: RRID:Addgene_139246 Copy
Species: Synthetic
Genetic Insert: TetON3G
Vector Backbone Description: Vector Backbone:pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Leonard Lab plasmid reference number: L1578
Proper citation: RRID:Addgene_139259 Copy
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